FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49341
Sequences flagged as poor quality0
Sequence length48-552
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGCACCGTAAACTGGTCCCTT764115.48610688879431No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT714014.470724144220831No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA712914.448430311505644No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGCCCTTGGGAGTATGTCAGGGTACGCACTCGT544211.029367057822096No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGTCCCTTGGGAGTATGTCAGGGTACGCACTCGT499310.119373340629496No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGCACCGTAAACTGGTCTCTT13092.6529660931071524No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGCTCTTGGGAGTATGTCAGGGTACGCACTCGT10142.05508603392716No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGTCTCTTGGGAGTATGTCAGGGTACGCACTCGT9101.8443079791654No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGCACCGTAAACTGGTCCCTTGGGAGTATGTCAGGGTACGCACTCGT8011.6233963640785554No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGCCCTTGGGAGTATGTCAGGGTACGCACTCGT6861.3903244766016094No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGGACCAGTTTACGGTGCCAGT6601.3376299629111692No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT5921.1998135424900185No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGTCCCTTGGGAGTATGTCAGGGTACGCACTCGT5921.1998135424900185No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGGCCAGTCATTTTCAGCAGGCCTACGCACTCGT5241.0619971220688675No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGGACCAGTCATTTTCAGCAGGCCTACGCACTCGT5181.049836849678766No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGGCACCGTAAACTGGTCCCT3810.7721772967714478No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGCTCTTGGGAGTATGTCAGGGTACGCACTCGT3780.7660971605763969No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG3660.7417766157961938No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGTCTCTTGGGAGTATGTCAGGGTACGCACTCGT2780.5634259540747046No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGCACCGTAAACTGGTCTCTTGGGAGTATGTCAGGGTACGCACTCGT2430.4924910317991123No Hit
ACGAGTGCGTACCCTGACATACTACCCAAGGAAAGTAAAGTTCCCATATT1710.34656776311789383No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGCACCGTAACTGGTCTCTTGGGAGTATGTCAGGGTACGCACTCGT1700.3445410510528769No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGGCCCTTGGGAGTATGTCAGGGTACGCACTCGT1670.33846091485782615No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGGTCCCTTGGGAGTATGTCAGGGTACGCACTCGT1550.3141403700776231No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGAGACCAGTTTACGGTGCCAGT1380.2796862649723354No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT1140.23104517541192923No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGCTTGGGAGTATGTCAGGGTACGCACTCGT1070.21685819095681075No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGAGCCAGTCATTTTCAGCAGGCCTACGCACTCGT1060.21483147889179385No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGAGACCAGTCATTTTCAGCAGGCCTACGCACTCGT1010.20469791856670924No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGCACCGTAACTGGTCCCTTGGGAGTATGTCAGGGTACGCACTCGT960.1945643582416246No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGCCCTTGGAGTATGTCAGGGTACGCACTCGT900.18240408585152307No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGCACCGTAAACTGGTCCCTTG890.18037737378650615No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGCCCTTGGGAGTATGTCAGGGTACGCACTCGGT890.18037737378650615No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGGCACCGTAAACTGGTCTCT730.1479499807462354No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGCCCTTGGAGTATGTCAGGGTACGCACTCGT660.1337629962911169No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGGCACCGTAAACTAGGTCCC650.1317362842261No Hit
ACGAGTGCGTAGGCCTGCTGAATGACTGGCACCGTAACTGGTCTCTTGGGAGTATGTCAGGGTACGCACTCGT590.11957601183599845No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGTCCCTTGGAGTATGTCAGGGTACGCACTCGT570.11552258770596462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)