FastQCFastQC Report
Sat 17 Dec 2022
EGAF00000133017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000133017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204322777
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86748134.2456416887873445Clontech SMART CDS Primer II A (100% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4010170.19626642016518794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)