FastQCFastQC Report
Fri 17 May 2024
EGAF00000133021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000133021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195431618
Total Bases19.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65133593.3328071816915523Clontech SMART CDS Primer II A (100% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3811740.19504213489139716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)