FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000146453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000146453
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences306183716
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA99005223.2335233660826037Illumina Paired End PCR Primer 2 (100% over 32bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC8090750.2642449476313756Illumina Paired End PCR Primer 2 (100% over 50bp)
GGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGG4081830.13331309885859507Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)