FastQCFastQC Report
Wed 15 May 2024
EGAF00000146459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000146459
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences102269776
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA8388110.8201944238149109Illumina Paired End PCR Primer 2 (100% over 32bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC1264710.12366410189458125Illumina Paired End PCR Primer 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)