FastQCFastQC Report
Wed 8 May 2024
EGAF00000168320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584062
Total Bases352.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT2110448.167141500474834TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2030077.856119551311075Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTAT463961.7954677558046208TruSeq Adapter, Index 16 (97% over 40bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACC213070.8245545192027127TruSeq Adapter, Index 2 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)