FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000168322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3275663
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2901148.856649783570532TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACG159230.48610006584926474Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGAAGATCGGAAGAGCACACGTCTGAACTCC35420.10813078146317251Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)