FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000168333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4660234
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2581165.538691833929369TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACA610561.3101488036866817Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT174050.3734790999765248TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)