FastQCFastQC Report
Wed 8 May 2024
EGAF00000168340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266848
Total Bases448.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACC59859714.02902095410945Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2953076.920963671543959TruSeq Adapter, Index 2 (100% over 50bp)
AAGCAGAAGATCGGAAGAGCACACGTCTGAACTCC70300.16475862275853276Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACC70000.16405552764007528TruSeq Adapter, Index 2 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)