FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000168341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4816988
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC253606552.64835619270798TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACG48839410.138991419534364Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTT288810.5995655376347211TruSeq Adapter, Index 6 (100% over 62bp)
AAGCAGAAGATCGGAAGAGCACACGTCTGAACTCC284930.5915107116729376Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACG262780.5455276201643019Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCATTATCTCGTATGC239040.49624371080019297TruSeq Adapter, Index 6 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC131800.27361496437192706TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGCCAATATCTCGTATGC123490.2563635201084163TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATGC114000.2366624122792085TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGC110440.2292719018606648TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATGC108980.22624096219463283TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGCCAATATCTCGTATGC107110.22235886823882478TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGC103520.21490607823810232TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGCCAATATCTCGTATGC99220.20597933812581637TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGC94820.19684499940626798TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGCCAATATCTCGTATGC76910.15966408884556077TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC76710.15924889163103584TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC68020.14120857265992776TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACGCCAATATCTCGTATG64730.1343785784809927Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG52580.10915534769860337TruSeq Adapter, Index 6 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)