FastQCFastQC Report
Wed 8 May 2024
EGAF00000168342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380645
Total Bases207.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACG46720619.625185611462438Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1707407.17200590596246TruSeq Adapter, Index 6 (100% over 50bp)
AAGCAGAAGATCGGAAGAGCACACGTCTGAACTCC81450.3421341695212852Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACG78380.3292385046909556TruSeq Adapter, Index 6 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)