FastQCFastQC Report
Wed 8 May 2024
EGAF00000168343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528566
Total Bases241.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACC42477816.799166009508948Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1856077.340405589571322TruSeq Adapter, Index 12 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTC75540.29874640408832515TruSeq Adapter, Index 12 (100% over 50bp)
GCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGC62220.2460683248924489TruSeq Adapter, Index 12 (100% over 50bp)
AAGCAGAAGATCGGAAGAGCACACGTCTGAACTCC47130.18639023066829183Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT45320.17923202320999332TruSeq Adapter, Index 12 (100% over 50bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACC39430.1559381878898949TruSeq Adapter, Index 2 (100% over 34bp)
GAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCT34280.13557091252512293TruSeq Adapter, Index 12 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTG28010.11077424911985687TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC26120.10329965680152307TruSeq Adapter, Index 12 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)