FastQCFastQC Report
Fri 10 May 2024
EGAF00000168344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830126
Total Bases327.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACA33182811.724848999655846Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2331078.236629747226802TruSeq Adapter, Index 14 (97% over 44bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACA72130.2548649777430404TruSeq Adapter, Index 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)