FastQCFastQC Report
Wed 8 May 2024
EGAF00000168345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721618
Total Bases284.1 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACC36730213.49572203005712Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1796266.599971046634759TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTAT265030.9737957347430829TruSeq Adapter, Index 16 (97% over 40bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACC77710.28552868183558455TruSeq Adapter, Index 2 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACA35810.13157614330886994Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)