FastQCFastQC Report
Wed 8 May 2024
EGAF00000168358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000168358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899512
Total Bases429.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC721152.4871426640069085TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC66330.2287626331603387TruSeq Adapter, Index 4 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACT53000.1827893797301063Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)