FastQCFastQC Report
Wed 15 May 2024
EGAF00000223554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223554
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences54454580
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1222970.22458533331815247Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG712610.1308631891018166No Hit
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT641380.11778256300939241No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT548350.10069860055848379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)