FastQCFastQC Report
Tue 4 Apr 2023
EGAF00000223583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223583
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences54925927
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC6487591.181152572991622TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC1643610.29924119441807506TruSeq Adapter, Index 1 (98% over 50bp)
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCACTCATCGACCT871290.15863000364108557No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT743560.13537504792590938No Hit
GTGGATTCTCGTTTTACACGATGAAATATGGGCTGAAAATAGGAAAAGGC552010.10050080720531125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)