FastQCFastQC Report
Tue 4 Apr 2023
EGAF00000223584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223584
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences54925927
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6004071.093121286783198Illumina Single End PCR Primer 1 (100% over 50bp)
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCACTCATCGACCT850700.15488131861661614No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT730400.1329790938257628No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG584320.10638327506061027Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)