FastQCFastQC Report
Tue 4 Apr 2023
EGAF00000223605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223605
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences53230934
Sequences flagged as poor quality0
Sequence length90
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC7308281.3729385248058956TruSeq Adapter, Index 12 (100% over 50bp)
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT646250.12140497102680933No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT561050.10539924022373906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)