FastQCFastQC Report
Tue 8 Aug 2023
EGAF00000223619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223619
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences52386543
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1154350.22035239088023045TruSeq Adapter, Index 5 (100% over 50bp)
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT821670.15684753239014074No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGGCCCCTAACAGGGGCC748010.14278666946967658No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA702440.13408787061975055No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG688550.13143642633567174No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT665960.1271242502105932No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC620990.11853998459108096No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT585400.11174625514037068No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGTATGGCT540530.10318107839259406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)