FastQCFastQC Report
Fri 15 Sep 2023
EGAF00000223625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223625
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences51266413
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT2351070.45859849800687247No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG2140690.4175618840350699No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT2071310.40402865712488994No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1690820.32981047454987733No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT1614650.31495279375211993No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA1325630.25857670206027483No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT1277740.24923530343345848No Hit
CATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCT1182240.23060712283498358No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC1002920.19562905639604627No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC929030.1812161112188598No Hit
GGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCT923120.18006330967606413No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC843350.16450341474056318TruSeq Adapter, Index 10 (100% over 50bp)
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT791620.15441298770015371No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA783640.1528564130281555No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA773840.15094483009763135No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC743310.1449896640906006No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT717730.14000004252296722No Hit
CTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTT711970.13887649990257753No Hit
GGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAAT675620.1317860877061947No Hit
CAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTC638230.12449281364779703No Hit
GCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGACCGGG637610.12437187676851899No Hit
GGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTT633880.1236443048980236No Hit
GGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGA632810.12343559125152759No Hit
GTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGG627880.12247394800178432No Hit
GTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGG615840.12012543182999755No Hit
CGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCA588440.11478080200383825No Hit
CGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAG587330.11456428597803399No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG572500.11167155384949597No Hit
CTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTC561510.10952785013455106No Hit
GTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGT517340.10091207278340306No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA517070.10085940672307228No Hit
GTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCAT515650.10058242225762899No Hit
GGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTC515120.10047904073179452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)