FastQCFastQC Report
Thu 5 Jan 2023
EGAF00000223629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223629
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences54308045
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC3072720.5657946258238535TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC1543530.2842175592953125TruSeq Adapter, Index 1 (98% over 50bp)
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG1289100.2373681468371767No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1145010.21083616617022394No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT1097680.20212106696162602No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC854460.1573358054041533No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA757470.13947657294605245No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC745200.13721723917699485No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT736990.13570549262084466No Hit
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC621580.11445449748743487No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA614890.11322263579917119No Hit
GTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGT579420.10669137509921411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)