FastQCFastQC Report
Fri 6 Jan 2023
EGAF00000223630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223630
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences54308045
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG1420240.2615155820836489No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1404610.25863755544873696Illumina Single End PCR Primer 1 (100% over 50bp)
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1354180.2493516384174757No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG979710.18039868678756527Illumina Single End PCR Primer 1 (98% over 50bp)
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT948060.17457082095295456No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC818830.15077508313915555No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT811870.14949350505988568No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA775380.14277442688279424No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC701250.12912451552988144No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA687210.12653926319756123No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT634000.11674145147371812No Hit
GTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGT622430.11461101205171352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)