FastQCFastQC Report
Fri 2 Jun 2023
EGAF00000223631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223631
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences54999695
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1407340.255881418978778TruSeq Adapter, Index 2 (100% over 50bp)
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT725180.13185164026818694No Hit
GGCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT712340.12951708186745398No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA652500.11863702153257397No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT583390.10607149730557595No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT573390.1042533054046936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)