FastQCFastQC Report
Mon 3 Apr 2023
EGAF00000223633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223633
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences53026947
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC4021510.7583898805262163TruSeq Adapter, Index 3 (100% over 50bp)
CGCAAAATTAACCCCCTAATAAAATTAATTAACCGCTCATTCATCGACCT1155750.2179552218987829No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT843050.15898520425850654No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT770140.14523559125514054No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA747590.14098303641731438No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC571780.10782819535131827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)