FastQCFastQC Report
Mon 3 Apr 2023
EGAF00000223635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223635
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences51944079
Sequences flagged as poor quality0
Sequence length90
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC9077701.7475909044416784TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC917510.1766341838499052TruSeq Adapter, Index 5 (98% over 50bp)
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT749820.14435138988603494No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA689270.13269462338527555No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT589940.11357213591177544No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT587390.11308122336715219No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)