FastQCFastQC Report
Mon 3 Apr 2023
EGAF00000223636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223636
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences51944079
Sequences flagged as poor quality0
Sequence length90
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7637191.4702715202631662Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG840260.16176242146867212Illumina Single End PCR Primer 1 (98% over 50bp)
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT693390.13348778404560796No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT605540.1165753655965293No Hit
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT603400.11616338408849256No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT586020.11281747819611933No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA556490.10713251841465896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)