FastQCFastQC Report
Mon 4 Sep 2023
EGAF00000223637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223637
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences55233804
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC11920132.1581222252952195TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC1213290.2196643924796489TruSeq Adapter, Index 8 (98% over 50bp)
GGGGAATGCTGGAGATTGTAATGGGTATGGAGACATATCATATAAGTAAT1139660.20633378791002696No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1044600.18912331296247492No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG839820.15204819135759687No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA821480.14872776099216342No Hit
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT807070.14611885141932285No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC752060.13615937080849982No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT749890.13576649545991798No Hit
GTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGG636100.11516498121331639No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT617950.11187895007195231No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG568520.10292972035748253No Hit
GTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGT559110.10122605352330975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)