FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000223742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223742
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences8168152
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG252517030.914826266700228RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA6314537.73067151541744RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC4201315.143525732625935RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC3285984.022917301245128RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCC1300941.5926980790759036RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1285171.5733913864482443RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1134871.3893840369278143RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCC1052701.2887860069205372RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC1027721.2582038140328438RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA978701.198190239358915RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC799710.979058665901418RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGGGCCAAGGAACTCCAG764490.9359399776106028RNA PCR Primer, Index 1 (96% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC731590.8956615890595573RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCC720890.8825619307769983RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA717690.8786442759635227RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC563550.689935740666922RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCA511240.6258943271378887RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC483920.5924473491678411RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA461630.5651584348577255RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC420140.5143635916667565RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGGGCCAAGGAACTCCA386780.47352204023627376RNA PCR Primer, Index 1 (96% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC374570.4585737385886061RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTC373860.4577045089268662RNA PCR Primer, Index 1 (100% over 26bp)
CTGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA372690.4562721163856892RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTC362740.4440906584500386RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG305900.37450331482567906RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTC303520.3715895590581566RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC287200.35160951950943126RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC279720.34245200138293214RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA276960.33907302410630946RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTC253450.3102905038985563RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTC252500.30912745012580567RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCC240640.2946076419733619RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC232070.28411567267602267RNA PCR Primer, Index 1 (100% over 31bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCC230780.28253636807934035RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCA224340.27465208776722083RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA213820.2617727975679199RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTC188080.23026016166202587RNA PCR Primer, Index 1 (100% over 26bp)
CCAGGGTTCAAGTCCCTGTTCGGGCGCTGGAATTCTCGGGTGCCAAGGA184180.22548551985810253RNA PCR Primer, Index 1 (100% over 22bp)
CGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG165840.20303246070837075Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACT161330.19751101595562864RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT152740.1869945613157052RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACT146970.17993053998015707RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACT144520.17693108551358985RNA PCR Primer, Index 1 (100% over 25bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCC142830.17486207406522308RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTACGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG138270.16927941595602042RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCC133870.16389264058749153RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA132850.1626438881156962RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG126620.15501670390071096RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG126240.15455148239161073RNA PCR Primer, Index 1 (100% over 29bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCTGGAATTCTCGGGTGCCAAGG125370.15348636998919707Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCC119860.14674065810724385RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA119850.14672841543595172RNA PCR Primer, Index 1 (100% over 28bp)
CGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG119410.14618973789909884Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCC118610.14521032419572996RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCCTGGAATTCTCGGGTGCCAAGGAACTCC114240.13986027684107738RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGTTCGAGCCCCACGTTGGGCGCTGGAATTCTCGGGTGCCAAGGA112510.13774229470754218RNA PCR Primer, Index 1 (100% over 22bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTC110610.13541618716204107RNA PCR Primer, Index 1 (100% over 26bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGTGGAATTCTCGGGTGCCAAGGA109770.13438780277350373RNA PCR Primer, Index 1 (100% over 22bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTC108750.1331390503017084RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCC107830.13201272454283416RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC107600.13173114310311562RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTC105860.1296009182982883RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA103640.1268830452714396RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCATGGAATTCTCGGGTGCCAAGGAACTCCAG103300.12646679444750783RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTC102370.1253282260173415RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG101240.12394480416133295RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC101180.12387134813358028RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA100940.12357752402256961RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGTTCGAGCCCCACGTTGGGCGTGGAATTCTCGGGTGCCAAGGAA97990.11996593599139682RNA PCR Primer, Index 1 (100% over 23bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTC97450.11930483174162282RNA PCR Primer, Index 1 (100% over 26bp)
CTGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCC97450.11930483174162282RNA PCR Primer, Index 1 (100% over 27bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCTGGAATTCTCGGGTGCCAAGG97260.11907222098707271Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC92430.11315901075298306RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC90480.11077168985102138RNA PCR Primer, Index 1 (100% over 26bp)
TCGCGGGTTCGATCCCCGTACGGGCCTGGAATTCTCGGGTGCCAAGGAA89730.10985348950411304RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACT88650.10853128100456505RNA PCR Primer, Index 1 (100% over 25bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA88630.10850679566198082RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCC87380.10697646175046693RNA PCR Primer, Index 1 (100% over 27bp)
CCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG86610.10603377606097437No Hit
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA85530.10471156756142637RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACT84200.1030832922795756RNA PCR Primer, Index 1 (100% over 25bp)
AACCCGTAGATCCGAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTC83400.10210387857620672RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCC82930.10152847302547748RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTATGGAATTCTCGGGTGCCAAGGAACTCCA82080.10048784596564805RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC81990.10037766192401905RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph