FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000223750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223750
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7122349
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG141707819.89621682397198RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC4016245.63892614641602RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA2924534.106131277756819RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC1736802.438521336149071RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCC1631852.291168264851947RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCC1464372.056021124491372RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC882801.239478716923307RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTC838721.177589022947345RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC789811.108917858420024RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG713321.001523514222625RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC691270.9705646269229435RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT672650.9444215665365457RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC662000.9294686345754751RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACT644270.904575161930425RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCC598160.8398352846792541RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA581070.8158403919830381RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA534260.7501176929128298RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC516450.7251118977741754RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC493360.692692818057638RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCA475650.667827426035989RNA PCR Primer, Index 1 (100% over 28bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA467810.6568198216627689RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA453620.6368966193597084RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGGGCCAAGGAACTCCAG420370.5902125829554267RNA PCR Primer, Index 1 (96% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG415280.5830660642998539RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCC412540.579219018893907RNA PCR Primer, Index 1 (100% over 27bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCTGGAATTCTCGGGTGCCAAGG405410.569208276651425Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC401510.563732555088216RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCC349640.49090545829753635RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC331000.46473431728773756RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTC329930.4632320039357801RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTC314630.4417503270339603RNA PCR Primer, Index 1 (100% over 26bp)
CGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG308120.4326100841169115Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCC301330.42307671247224754RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA298090.4185276514812739RNA PCR Primer, Index 1 (100% over 22bp)
CCAGGGTTCAAGTCCCTGTTCGGGCGCTGGAATTCTCGGGTGCCAAGGA294390.41333273615207566RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT288560.40514723443066325RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTC284940.4000646415950692RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCACAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC266060.3735565331044575RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTACGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG260320.3654973941883499RNA PCR Primer, Index 1 (100% over 29bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCTGGAATTCTCGGGTGCCAAGG248400.3487613426413112Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCC244520.3433137017015032RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC236520.33208145234107456RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA234300.3289645031435556RNA PCR Primer, Index 1 (100% over 28bp)
TCGCGGGTTCGATCCCCGTACGGGCCTGGAATTCTCGGGTGCCAAGGAA233570.32793956038941646RNA PCR Primer, Index 1 (100% over 23bp)
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACT224210.3147978286377149RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA223490.3137869261952763RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACT211700.2972333987003445RNA PCR Primer, Index 1 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA206260.289595469135253No Hit
CGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG201000.2822102651807711Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG195630.27467061779758334No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACT186580.2619641357085984RNA PCR Primer, Index 1 (100% over 25bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGTGGAATTCTCGGGTGCCAAGGA183820.25808900967925047RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC183010.2569517444315071RNA PCR Primer, Index 1 (100% over 27bp)
CTGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA177060.24859775896968825RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA175300.2461266641103939RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA169950.23861509735060724RNA PCR Primer, Index 1 (100% over 28bp)
TTGGTCGTGGTTGTAGTCCGTGCGAGAATGGAATTCTCGGGTGCCAAGG163600.22969949942076695Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGG161810.227186283626371Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCC158520.22256702107689474RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGATCTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC158500.22253894045349362RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGTTCGAGCCCCACGTTGGGCGCTGGAATTCTCGGGTGCCAAGGA157720.22144379614085186RNA PCR Primer, Index 1 (100% over 22bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAA154880.21745634761789964No Hit
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACT154260.21658584829246644RNA PCR Primer, Index 1 (100% over 25bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCC154190.2164875661105627RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCA152600.21425515655017746RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGGGCCAAGGAACTCCA148230.2081195403370433RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA146900.20625217888087205RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTC144630.2030650281248504RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACT144610.20303694750144932RNA PCR Primer, Index 1 (100% over 25bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGTGGAATTCTCGGGTGCCAAGGA141990.19935838583590892RNA PCR Primer, Index 1 (100% over 22bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC134760.18920724047642148RNA PCR Primer, Index 1 (100% over 27bp)
TCGCGGGTTCGATCCCCGTACGGGCCATGGAATTCTCGGGTGCCAAGGA128980.18109194031351172RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC128620.18058648909229244RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGC128520.18044608597528708No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTC119970.1684416194713289RNA PCR Primer, Index 1 (100% over 26bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTC119840.16825909541922196RNA PCR Primer, Index 1 (100% over 26bp)
ACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC118690.16664445957366034RNA PCR Primer, Index 1 (100% over 26bp)
CGTGGGTTCGAGCCCCACGTTGGGCGTGGAATTCTCGGGTGCCAAGGAA117370.16479113842918958RNA PCR Primer, Index 1 (100% over 23bp)
TACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACT116680.16382235692185262RNA PCR Primer, Index 1 (100% over 25bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACT114950.1613933829976599RNA PCR Primer, Index 1 (100% over 25bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAG114880.16129510081575615No Hit
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTC114440.16067732710093258RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT110230.15476635587500698RNA PCR Primer, Index 1 (100% over 25bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC110080.15455575119949894RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTC109790.1541485821601834RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC109520.15376949374426893RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTC105400.14798488532364815RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA105170.1476619581545358RNA PCR Primer, Index 1 (100% over 28bp)
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAG102230.14353410651457826No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCC101000.14180714817541235RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT97260.13655607159941194RNA PCR Primer, Index 1 (97% over 35bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTC90530.12710694182495128RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACT87640.12304929174349642RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCC86830.121912026495753RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGCAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC86750.12179970400214873RNA PCR Primer, Index 1 (100% over 27bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC85500.12004466503958175RNA PCR Primer, Index 1 (100% over 31bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAA84460.118584472622726No Hit
TCAGTGCACTACAGAACTTTGTTTGGAATTCTCGGGTGCCAAGGAACTC84260.11830366638871528RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTCACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTC83080.11664690960805206RNA PCR Primer, Index 1 (100% over 26bp)
TTTGTTCGTTCGGCTCGCGTGAATGGAATTCTCGGGTGCCAAGGAACTC82840.1163099421272392RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTACGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA82010.11514459625609473RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGATCTTGTGATGGAATTCTCGGGTGCCAAGGAACTC81420.11431621786576311RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG80590.11315087199461862RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG80260.11268754170850094RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGATGGAATTCTCGGGTGCCAAGGAACTCC80170.11256117890319611RNA PCR Primer, Index 1 (100% over 27bp)
CCAGGGTTCAAGTCCCTGTTCGGGCGTGGAATTCTCGGGTGCCAAGGAA78540.11027260809600876RNA PCR Primer, Index 1 (100% over 23bp)
GCGGGTGATGCGACCTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA78090.10964079406948467RNA PCR Primer, Index 1 (100% over 22bp)
AACCCGTAGATCCGAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTC77500.10881241567915305RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACT75410.10587799053374104RNA PCR Primer, Index 1 (100% over 25bp)
AACCCGTAGATCCGATCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG74290.10430547562328102RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCC74080.10401062907756976RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATCGTTTGGAATTCTCGGGTGCCAAGGAACTCC74050.10396850814246818RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTC73990.10388426627226495RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATG72940.10241003354370867RNA PCR Primer, Index 17 (100% over 49bp)
CGCGGGTTCGATCCCCGTACGGGCCTGGAATTCTCGGGTGCCAAGGAAC72530.10183438076398672RNA PCR Primer, Index 1 (100% over 24bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA72400.10165185671187976RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG72330.101553574529976RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph