FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000223751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223751
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences8606973
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG281417932.69650084878853RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA7072468.217128135524533RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC5095265.919920975701911RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC3498634.064878558350305RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCC1600701.8597711413757194RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCC1462671.6994011715849462RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA1179071.3699008931479162RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1127691.3102051092759324RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGGGCCAAGGAACTCCAG1093391.270353700424063RNA PCR Primer, Index 1 (96% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG995221.1562950180045877RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTC943521.0962274425631404RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC776760.9024775609264721RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC723140.8401792360682437RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCC665640.7733729384302704RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC553030.6425371614387544RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGGGCCAAGGAACTCCA513150.5962026370943652RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC446740.5190442679441425RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC442310.5138972784043822RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCC427800.49703885442652135RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA408250.47432471322961045RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC400580.4654133340490321RNA PCR Primer, Index 1 (100% over 27bp)
CTGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA386720.4493101116966441RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCA366240.4255154512509799RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG317070.3683873529056034RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACT283710.32962808178903313RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC276290.3210071647720982RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA276030.320705084121909RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTC271800.3157904643130634RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC269190.31275803932462665RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCC268910.3124327217013461RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA266760.3099347470940132RNA PCR Primer, Index 1 (100% over 22bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA250180.2906712964011854RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACT247290.2873135537894681RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACT240770.2797383005616493RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC235740.2738942018291448RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTC235540.27366183209823014RNA PCR Primer, Index 1 (100% over 26bp)
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC232010.2695605063475858RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT220940.2566988417414578RNA PCR Primer, Index 1 (100% over 25bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA213790.2483916238612576RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTC210990.24513844762845197RNA PCR Primer, Index 1 (100% over 26bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCTGGAATTCTCGGGTGCCAAGG165660.1924718481166375Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCC163140.18954398950711243RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCC161870.18806844171580414RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTC158880.18459451423862955RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA157300.1827587933644035RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA149340.17351047807399883RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA147990.17194198239032468RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCC146240.16990874724482113RNA PCR Primer, Index 1 (100% over 27bp)
CCAGGGTTCAAGTCCCTGTTCGGGCGCTGGAATTCTCGGGTGCCAAGGA143740.1670041256083875RNA PCR Primer, Index 1 (100% over 22bp)
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACT142640.16572609208835673RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG140030.16269366709992003RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCCTGGAATTCTCGGGTGCCAAGGAACTCC139500.1620778873129961RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACT137450.15969609757112052RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCC124990.14521946333513536RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTACGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG121260.1408857678535764RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGG121170.1407812014746648Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGGGCCAAGGAACTCC117180.1361454253429167RNA PCR Primer, Index 1 (96% over 27bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGTGGAATTCTCGGGTGCCAAGGA112510.13071959212605871RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTC112080.13021999720459213RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCA111020.12898843763074427RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCTGGAATTCTCGGGTGCCAAGG108680.1262697117790424Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA105640.12273769186913913RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTC104370.12126214407783084RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCATGGAATTCTCGGGTGCCAAGGAACTCCAG102410.11898492071486688RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGCGCCAAGGAACTCC101720.1181832451432112RNA PCR Primer, Index 1 (96% over 27bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACT101680.11813677119702827RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGGGCCAAGGAACTCCA98000.113861168148198RNA PCR Primer, Index 1 (96% over 28bp)
CTGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCC97370.1131292034958167RNA PCR Primer, Index 1 (100% over 27bp)
GATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA93340.1084469534178857RNA PCR Primer, Index 1 (100% over 32bp)
TGAGATGAAGCACTGTAGCTATGGAATTCTCGGGTGCCAAGGAACTCCA92300.10723863081712932RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC90900.10561204270072648RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCC89500.10398545458432368RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC89110.10353233360904002RNA PCR Primer, Index 1 (100% over 31bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC88590.10292817230866183RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGTTCGAGCCCCACGTTGGGCGCTGGAATTCTCGGGTGCCAAGGA88570.10290493533557037RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTC87820.10203354884464028RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT87590.10176632365408837RNA PCR Primer, Index 1 (100% over 25bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC86870.1009297926227955RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph