FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000223756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223756
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7642748
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG177105723.173039330879416RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC4520135.914273243079584RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA4169445.455419961511225RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC2512183.287011425733257RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCC1961392.566341321210643RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCC1854502.4264832492187365RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTC1396281.8269345005225868RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC1114821.4586638209188632RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1096141.4342223503901999RNA PCR Primer, Index 1 (100% over 30bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACT963251.2603451010029376RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCC848331.1099803369154655RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC824101.0782770804427937RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC694260.9083905422499865RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG686690.8984857279083387RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA680910.8909230030873713RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA677350.886264992644007RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC633080.8283408009789149RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC602600.7884598576323595RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA601750.7873476922175113RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACT538130.7041053819908756RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC529580.6929183063474028RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCC472600.618363970655581RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCA412380.5395703220883379RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACT403710.5282262348568866RNA PCR Primer, Index 1 (100% over 25bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTC384920.5036408370392429RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCC367760.48118817995830815RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGG353720.4628178241648161Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTC350530.4586439327843859RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCC340890.4460306685501079RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC332300.4347912557106423RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC318870.4172190421560413RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCC315090.41227317713471645RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC314180.41108250592587897RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTC305660.39993468317940095RNA PCR Primer, Index 1 (100% over 26bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA289860.3792614907622232RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG280760.36735477867384875RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA268200.3509208991320923RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACT265600.3475189813925567RNA PCR Primer, Index 1 (100% over 25bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA256940.3361879784601036RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTACGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG248120.3246476267436791RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG240260.3143633677310831Illumina Small RNA Adapter 2 (100% over 21bp)
CAACGGAATCCCACAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC238740.3123745542833546RNA PCR Primer, Index 1 (100% over 26bp)
CTGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA231670.30312395489161753RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT216810.28368068658027196RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC210510.27543757821139725RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACT207420.2713945298209492RNA PCR Primer, Index 1 (100% over 25bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA201430.2635570347210192RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACT185470.24267449351987008RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTC184470.2413660636200487RNA PCR Primer, Index 1 (100% over 26bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCC181900.23800339877750776RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC177690.23249490889925978RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT164890.21574700618154624RNA PCR Primer, Index 1 (100% over 25bp)
GCGGGTGATGCGACCTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA161060.21073571966523036RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA158780.2077524994936376RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA154140.20168138475846645RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA153780.20121034999453077RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC147910.1935298664825793RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTC145220.19001019005205982RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTC144990.1897092511751009RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT141070.18458020596780111RNA PCR Primer, Index 1 (97% over 35bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTC137190.17950349795649417RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCA136490.17858759702661922RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACT134580.17608849591796039RNA PCR Primer, Index 1 (100% over 25bp)
TACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACT133540.17472772882214616RNA PCR Primer, Index 1 (100% over 25bp)
ACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC129080.16889213146894286RNA PCR Primer, Index 1 (100% over 26bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC124320.16266400514579313RNA PCR Primer, Index 1 (100% over 26bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTC123710.16186586290690205RNA PCR Primer, Index 1 (100% over 26bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAG117070.15317788837208815No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG116420.15232740893720426Illumina Small RNA Adapter 2 (100% over 21bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAG114980.15044326988146148No Hit
TTTGGCAATGGTAGAACTCACTGGAATTCTCGGGTGCCAAGGAACTCCA114950.15040401698446684RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA113780.14887315400167583No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCC113450.1484413721347348RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGTGATGCGCACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGG105830.1384711362980959Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC100600.13162804792203014RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTC100540.13154954212804087RNA PCR Primer, Index 1 (100% over 26bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC96490.12625040103376428RNA PCR Primer, Index 1 (100% over 31bp)
AACCCGTAGATCCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG94190.12324101226417514RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTC94120.12314942217118764RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTC93600.12246903862328054RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACT92340.1208204169495056RNA PCR Primer, Index 1 (100% over 25bp)
CACCATGATGGAACTGAGGATCTGAGGTGGAATTCTCGGGTGCCAAGGA91940.12029704498957704RNA PCR Primer, Index 1 (100% over 22bp)
AAGCTGCCAGTTGCAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC91610.119865263122636RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCA90940.11898861508975568RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTACGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA88870.11628016519712543RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATCGTTTGGAATTCTCGGGTGCCAAGGAACTCC87060.11391190707844875RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACT86280.11289133175658808RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTC85610.11201468372370776RNA PCR Primer, Index 1 (100% over 26bp)
TTTGTTCGTTCGGCTCGCGTGAATGGAATTCTCGGGTGCCAAGGAACTC85250.11154364895977206RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC83780.10962025700703465RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACTGGTGGAATTCTCGGGTGCCAAGGAA82110.10743517907433293RNA PCR Primer, Index 1 (100% over 23bp)
TCAGTGCACTACAGAACTTTGTTTGGAATTCTCGGGTGCCAAGGAACTC81630.10680713272241868RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCC80900.10585197889554909RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCC78220.10234538676402781RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCC77850.1018612677010939RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC77740.10171734041211355RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph