FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000223761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223761
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences8803733
Sequences flagged as poor quality0
Sequence length49
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG239386927.191522050929983RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC97613511.087739712233436RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA3515843.9935786330639518RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC2280102.589924069709974RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCC2267932.5761003883239075RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCC2021922.2966621091302972RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTC1971042.2388684436477115RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACT1283581.4579951481945215RNA PCR Primer, Index 1 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC1106101.256398848079559RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1100811.2503900334096911RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCC876130.9951801127998771RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC823180.9350351720116911RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA769270.8737997847049656RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG766140.8702444747018111RNA PCR Primer, Index 1 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC709580.8059990006512011RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC697350.7921071663577257RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC603470.6854705838988984RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTC596450.6774966937320793RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC539540.6128536610549183RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTC487830.5541172136865123RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCC483420.5491079749919722RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACT473800.5381807921707757RNA PCR Primer, Index 1 (100% over 25bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA446260.5068986076701781RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCA443010.5032069918522063RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC426200.48411281895986624RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA419590.4766046403270067RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCC417330.4740375474812787RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACT410700.46650665121261625RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCC373010.42369526654204526RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC371500.4219800850389261RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA370150.4204466446222302RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCACAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC369150.419310762832085RNA PCR Primer, Index 1 (100% over 26bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC363660.4130747718041881RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACT326440.3707972515749853RNA PCR Primer, Index 1 (100% over 25bp)
CTGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA296960.33731145640150606RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGG290890.33041665393532493Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA290310.32975784249704077RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTACGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG271430.3083123942991002RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTC268390.3048593136570589RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACT259770.2950680126260076RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC247650.28130112532944834RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGGGCCAAGGAACTCCAG240070.272691141360148RNA PCR Primer, Index 1 (96% over 29bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC236240.26834071410389204RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA223240.253574250832005RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACT217220.24673624245533116RNA PCR Primer, Index 1 (100% over 25bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA217170.2466794483658239RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCC214990.2442032260633075RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC211330.24004589871137616RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCC206930.2350480188347375RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT204640.2324468495353051RNA PCR Primer, Index 1 (100% over 25bp)
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACT203570.23123145601984976RNA PCR Primer, Index 1 (100% over 25bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA198230.22516584726047462RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC195140.22165597252892608RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC187930.2134662648219795RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTC175430.199267742445165RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA164710.18709108965480895RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACT159780.1814911924293933RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGCAATGGTAGAACTCACTGGAATTCTCGGGTGCCAAGGAACTCCA158010.17948068166083636RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG156220.17744745325647654RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA153340.1741761137008585RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTC150110.17050721551868964RNA PCR Primer, Index 1 (100% over 26bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACT146610.16653162925318157RNA PCR Primer, Index 1 (100% over 25bp)
TTTGTTCGTTCGGCTCGCGTGAATGGAATTCTCGGGTGCCAAGGAACTC136940.15554765234247792RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTC132270.15024308438250003RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTC128660.14614255112007601RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCA128530.14599488648735712RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA122340.1389637782063586RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG116910.1327959400858704RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCC115980.1317395700210354RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCC114100.12960411225556248RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC113580.129013453724687RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACTGGTGGAATTCTCGGGTGCCAAGGAA107420.12201642189739285RNA PCR Primer, Index 1 (100% over 23bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCC106560.12103956355786802RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTC104210.11837024135102688RNA PCR Primer, Index 1 (100% over 26bp)
GCGGGTGATGCGACCTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA103530.11759784173372817RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTC89790.10199082593713371RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTC89280.10141152622415968RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTC88840.10091173823649582RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTC88620.10066184424266389RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTATGGAATTCTCGGGTGCCAAGGAACTC88520.10054825606364937RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph