FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000223773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223773
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences9988222
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC114267211.440194260800371RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG8445418.455368733294073RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC2894282.897692902700801RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC1910301.9125526044575303RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA1791051.7931619861873316RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACT1748771.7508321300828116RNA PCR Primer, Index 1 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC1529191.5309932037954304RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCC1430761.43244713623706RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCA1286471.287986991078092RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTC1211441.2128685165387794RNA PCR Primer, Index 1 (100% over 26bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCC1205021.2064409461463712RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCC1140301.1416446290440883RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC1118791.1201092646919542RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC1109881.111188758119313RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCC931490.9325884026206065RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC884180.8852226151961782RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT860850.8618651047203395RNA PCR Primer, Index 1 (100% over 25bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG827370.8283456254776876Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTC816390.8173526779841298RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA812460.8134180437719546RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC761360.7622577872217898RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACT755320.7562106649211442RNA PCR Primer, Index 1 (100% over 25bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA665760.6665450567678612RNA PCR Primer, Index 7 (100% over 35bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC653890.654661059796228RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACT650770.6515373807270203RNA PCR Primer, Index 1 (100% over 25bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC616840.6175673708493864RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTC615220.6159454605634517RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTC610350.6110697179137589RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACT580770.5814548375076165RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT546560.547204497457105RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCC530250.5308752648869839RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA519900.5205130602824006RNA PCR Primer, Index 1 (100% over 22bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC500680.50127039627273RNA PCR Primer, Index 1 (100% over 31bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA500060.5006496651756439RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC496700.4972857031011125RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTC483020.48358957179766326RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA477260.47782277966989517RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA468780.46933278014845886RNA PCR Primer, Index 1 (100% over 32bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCC443740.4442632532596893RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTC432980.4334905651876781RNA PCR Primer, Index 1 (100% over 26bp)
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC431270.4317785487747469RNA PCR Primer, Index 1 (100% over 27bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA417780.41827264151717897RNA PCR Primer, Index 1 (100% over 32bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG409480.40996285424973533RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC405580.40605825541322565RNA PCR Primer, Index 1 (100% over 33bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTC404690.4051672059351504RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATG395060.3955258503465382RNA PCR Primer, Index 35 (100% over 49bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTC373910.3743509105023897RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCC359870.360294354690955RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA349380.3497919849999329RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG339840.340240735538317RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCC333710.33410350711067494RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACTTGGACTTCTCGGGTGCCAAGGAACT323200.3235811138358759RNA PCR Primer, Index 1 (96% over 25bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA318250.3186252768510752RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCA315400.3157719161628566RNA PCR Primer, Index 1 (100% over 28bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAG311560.3119273880776779No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT283120.283453851946823RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTC282420.28275302651462897RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGG279810.280139948831734Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACT279680.2800097955371837RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA276680.2770062579706378RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC271530.2718501851480674RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAA262050.2623590064377824No Hit
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA245920.2462099861216541RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC228050.22831891401692914RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACACTGGTGGAATTCTCGGGTGCCAAGGAA226730.22699735748764896RNA PCR Primer, Index 1 (100% over 23bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCA221750.22201148512718277RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACT221690.22195141437585186RNA PCR Primer, Index 1 (100% over 25bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCC220300.22055977530335227RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCC217910.2181669570420041RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG217430.21768639103135673RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGC212970.21322113184909186No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTC209870.21011747636366113RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACT207220.20746435151321224RNA PCR Primer, Index 1 (100% over 25bp)
TATGGCACTGGTAGAATTCACTTGGAATTCTCGGGTGCCAAGGAACTCC200690.20092665141003074RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCA194690.19491957627693898No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAG193880.1941086211339716No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCC181620.18183416427868743RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAAGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCA178980.17919105122012707RNA PCR Primer, Index 1 (100% over 28bp)
TATGGCACTGGTAGAATTCACTGTGGAATTCTCGGGTGCCAAGGAACTC178880.1790909333012422RNA PCR Primer, Index 1 (100% over 26bp)
TAATACTGCCTGGTAATGATGACTGGAATTCTCGGGTGCCAAGGAACTC176620.17682826833444432RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACTGGAATTCTCGGGTGCCAAGGAACTCCA175130.17533651134305986RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC172100.1723029384008485RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACT168120.16831824522923097RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGCAATGGTAGAACTCACACTGTTGGAATTCTCGGGTGCCAAGGAA167470.16766747875647936RNA PCR Primer, Index 1 (100% over 23bp)
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACT166070.1662658278920913RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC161020.16120987298840575RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCC160230.16041894142921534RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCA153200.15338065173160947RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTC152050.15222929566443358RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC150520.15069749150549516RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCC150030.15020691370295933RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTC149290.14946604110321135RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC148830.149005498676341RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA148380.14855496804135912RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGATTGGAATTCTCGGGTGCCAAGGAACTC146930.1471032582175286RNA PCR Primer, Index 1 (100% over 26bp)
TGTAACCATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT143680.14384942585377058RNA PCR Primer, Index 1 (100% over 25bp)
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT140690.1408559000791132RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTTGGACTTCTCGGGTGCCAAGGAACT140300.1404654401954622RNA PCR Primer, Index 1 (96% over 25bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTC140260.14042539302790827RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTC137460.13762209129913214RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA137360.13752197338024727RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC136360.13652079419139862RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTTGGACTTCTCGGGTGCCAAGGAACT135910.13607026355641677RNA PCR Primer, Index 1 (96% over 25bp)
TTTGGCAATGGTAGAACTCACACTGTGGAATTCTCGGGTGCCAAGGAAC127460.12761029941064586RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG126890.12703962727300214RNA PCR Primer, Index 1 (100% over 29bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA126790.1269395093541173RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCATCACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACT126500.12664916738935117RNA PCR Primer, Index 1 (100% over 25bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA126250.12639887259213903RNA PCR Primer, Index 1 (100% over 28bp)
TGTGCAAATCCATGCAAAACTGTGGAATTCTCGGGTGCCAAGGAACTCC126200.1263488136326966RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGACTTCTCGGGTGCCAAGGAACT121560.12170334219643895RNA PCR Primer, Index 1 (96% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGGGCCAAGGAACTCCAG121540.121683318612662RNA PCR Primer, Index 1 (96% over 29bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTC115560.11569626706334721RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGATGGAATTCTCGGGTGCCAAGGAACTCC111780.1119118097294994RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC110470.1106002649921077RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAAGCTCCTTGAGGTTGGAATTCTCGGGTGCCAAGGAACTCC110020.11014973435712581RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC107200.1073264090445727RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCC104640.10476339032112021RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTC104410.10453311910768504RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTC104320.10444301298068867RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT104310.10443300118880017RNA PCR Primer, Index 1 (100% over 25bp)
ATGGCACTGGTAGAATTCACTGTGGAATTCTCGGGTGCCAAGGAACTCC103500.10362204604583278RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTGTTGGACTTCTCGGGTGCCAAGGAACT103430.10355196350261338RNA PCR Primer, Index 1 (96% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAG101510.10162969946002401No Hit
CCACCCCGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA100050.10016797784430502RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph