FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000223776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223776
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences8438409
Sequences flagged as poor quality0
Sequence length49
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG8394979.948522286606396RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAA2955953.5029707614314503No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTC2543643.014359697426375RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC2202442.6100180733121614RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA1873502.220205254331711RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC1793132.124962181852053RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1569491.8599359191999347RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCC1493001.7692908698784333RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC1313151.556158275807679RNA PCR Primer, Index 1 (100% over 27bp)
CGGTGAAGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG1165961.3817296601764621RNA PCR Primer, Index 3 (97% over 37bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC1120031.3272999685130218RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA966251.1450618238580281RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC818250.9696733116396704RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC815910.9669002770545965RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCA791780.9383048392179141RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT790010.9362072874163838RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC784660.9298672297111932RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTC697530.82661316843021RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCC534670.6336147015391171RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGA510290.6047229993236877RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGC496350.5882032975647423No Hit
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC493890.5852880560778696RNA PCR Primer, Index 1 (100% over 24bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTC475150.5630800782469776RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA444930.527267640144013RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG409000.4846885236304616RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACT407060.48238951205138314RNA PCR Primer, Index 1 (100% over 25bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT382850.45369926961350177RNA PCR Primer, Index 1 (97% over 35bp)
AGTTGGTCCGAGTGTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC373890.44308115427920125RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCC364000.43136093545596094RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA354900.42057691206956194RNA PCR Primer, Index 1 (100% over 28bp)
GGGGATGTAGCTCAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT348750.4132888083523802RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA318640.37760672657606426RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC299630.35507878321612524RNA PCR Primer, Index 1 (100% over 27bp)
CGATGCCGACGCTCATCAGACTGGAATTCTCGGGTGCCAAGGAACTCCA297360.35238870265710037RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC285020.33776509292213736RNA PCR Primer, Index 1 (100% over 31bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTC284140.3367222423089471RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC282520.33480244913466506RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGG267540.3170502875601313Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAG248400.29436828672324367No Hit
CGGTGCAGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG242200.2870209301303125RNA PCR Primer, Index 3 (97% over 37bp)
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTC239530.2838568265652921RNA PCR Primer, Index 1 (100% over 26bp)
CACAAATGATGAATAACAAAGGGACTTTGGAATTCTCGGGTGCCAAGGA238540.2826836196254531RNA PCR Primer, Index 1 (100% over 22bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC233540.2767583320505086RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCA215000.2547873657226143No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA214300.2539578254621221RNA PCR Primer, Index 1 (100% over 32bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCC211120.25018934256445735RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTC202690.24019930771310088RNA PCR Primer, Index 1 (100% over 26bp)
AGTTGGTCCGAGTGTTGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC193880.22975895100604865RNA PCR Primer, Index 1 (100% over 26bp)
CACGGTGAAGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT182890.2167351689163206RNA PCR Primer, Index 1 (97% over 35bp)
CTGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA181500.215087938970486RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCC175610.20810795020720138RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACT169870.2013057200711651RNA PCR Primer, Index 1 (100% over 25bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT168510.19969404185078019RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTC167030.1979401567285966RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACT163630.19391096117763432RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG162130.19213337490515095RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC153980.1824751561579914RNA PCR Primer, Index 1 (100% over 24bp)
ACAAATGATGAATAACAAAGGGACTTTGGAATTCTCGGGTGCCAAGGAA153700.18214334005379448RNA PCR Primer, Index 1 (100% over 23bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCC149440.17709499503994178RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACTGGAATTCTCGGGTGCCAAGGAACTCCA149150.176751328360595RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGTGGAATTCTCGGGTGCCAAGGAACTCC146640.17377683399797283RNA PCR Primer, Index 1 (100% over 27bp)
CCCGAGATTGAGCAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC145330.17222440865333738RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCC145090.17193999484974004RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT144510.17125266149104648RNA PCR Primer, Index 1 (100% over 30bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA135860.16100191398639246RNA PCR Primer, Index 1 (100% over 22bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCC132730.1572926839644772RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC130960.15519513216294684RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA127480.15107113201078542RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTC127050.15056155727934023RNA PCR Primer, Index 1 (100% over 26bp)
AACGAGACTCTGGCATGCTAACTTGGAATTCTCGGGTGCCAAGGAACTC125520.1487484192814072RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA120920.14329715471245824RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGT120260.14251501675256556RNA PCR Primer, Index 1 (100% over 30bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA119520.14163807419147376RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAA118680.1406426258788831RNA PCR Primer, Index 1 (100% over 23bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACT116180.13767998209141083RNA PCR Primer, Index 1 (100% over 25bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA112720.13357968308954923RNA PCR Primer, Index 1 (100% over 28bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA112350.13314121180900335RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC109640.1299297059433834RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCC108860.12900536108169208RNA PCR Primer, Index 1 (100% over 27bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTC108720.1288394530295936RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCC106260.1259242115427209No Hit
CCGAGATTGAGCAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC106240.12590051039242114RNA PCR Primer, Index 2 (100% over 34bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTC105960.12556869428822423RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCC104980.1244073379235351RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCC104920.12433623447263577RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC104340.12364890111394222RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT104300.12360149881334266RNA PCR Primer, Index 1 (100% over 30bp)
GACTCTTAGCGGTGGATCACTCGGCTGGCATTCTCGGGTGCCAAGGAAC99510.1179250733165458RNA PCR Primer, Index 1 (95% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATG98450.11666891235065757RNA PCR Primer, Index 38 (100% over 49bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCA98110.11626599279556135RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTC97980.11611193531861279RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC97510.115554958286568RNA PCR Primer, Index 1 (100% over 31bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGG97500.11554310771141811Illumina Small RNA Adapter 2 (100% over 21bp)
ACGAGAACTTTGAAGGCCGAAGTTGGAATTCTCGGGTGCCAAGGAACTC97230.1152231421823711RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACTGGAATTCTCGGGTGCCAAGGAA97140.1151164870060221RNA PCR Primer, Index 1 (100% over 23bp)
AACCCGTAGATCCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG96930.11486762492787443RNA PCR Primer, Index 1 (100% over 29bp)
AACGAGAACTTTGAAGGCCGAAGTTGGAATTCTCGGGTGCCAAGGAACT93560.11087398110236184RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT90780.10757952121069267RNA PCR Primer, Index 1 (100% over 25bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA90670.1074491648840439RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCTCGGGTGCCAAGG88180.10449837167172153Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGCTGAGAAGACGGTCGAACTGGAATTCTCGGGTGCCAAGGAACTCC87620.10383473946332775RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph