FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000223778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223778
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences9429495
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAA8266888.767044258467713No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCA4989395.291258969859998No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC4230254.486189345240652RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2920663.09736629586208RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTC2225592.36024304588952RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1502581.5934893650190174RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTC1398391.4829956429267952RNA PCR Primer, Index 1 (100% over 26bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1238931.3138879653682407RNA PCR Primer, Index 1 (100% over 31bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC1214911.2884147030143183RNA PCR Primer, Index 1 (100% over 27bp)
CGGTGAAGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA1184101.2557406308609316RNA PCR Primer, Index 1 (97% over 37bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC977911.0370756864498045RNA PCR Primer, Index 1 (100% over 31bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC958821.016830699841296RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT943771.0008701420383594RNA PCR Primer, Index 1 (97% over 35bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA867680.9201765311928157RNA PCR Primer, Index 1 (100% over 32bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCC844970.8960925266941654RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGC684970.7264121779586287No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA682070.7233367216377972RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCC639020.6776821028061418RNA PCR Primer, Index 1 (100% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAAC601750.6381571865725577RNA PCR Primer, Index 1 (100% over 24bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCA586640.6221329986388455RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACT567160.6014744161802938RNA PCR Primer, Index 1 (100% over 25bp)
AGTTGGTCCGAGTGTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC496880.5269423229982094RNA PCR Primer, Index 1 (100% over 31bp)
CACAAATGATGAATAACAAAGGGACTTTGGAATTCTCGGGTGCCAAGGA491330.5210565359014454RNA PCR Primer, Index 1 (100% over 22bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTC488460.5180128946460018RNA PCR Primer, Index 1 (100% over 26bp)
CGGTGAAGAGACATGAGAGGTTGGAATTCTCGGGTGCCAAGGAACTCCA479280.5082774846373003RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATG475350.5041097110714837RNA PCR Primer, Index 40 (100% over 49bp)
CGACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAA464150.4922320866599961RNA PCR Primer, Index 1 (100% over 23bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC454010.4814785945588815RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTC453680.48112862883961444RNA PCR Primer, Index 1 (100% over 26bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA449580.47678056990326634RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCC447480.4745535153261124RNA PCR Primer, Index 1 (100% over 27bp)
CGATGCCGACGCTCATCAGACTGGAATTCTCGGGTGCCAAGGAACTCCA425510.451254282440364RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAATAACAAAGGGACTTTGGAATTCTCGGGTGCCAAGGAA421490.44699106367838365RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGA394820.4187074705485288RNA PCR Primer, Index 1 (100% over 22bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC382290.40541937823817714RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC370360.39276758723558364RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTG359900.38167473443699795No Hit
GGGGATGTAGCTCAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT339670.3602207753437485RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGC294300.3121057914554279No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGA283720.3008856783952905RNA PCR Primer, Index 1 (100% over 22bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT264670.28068311187396566RNA PCR Primer, Index 1 (100% over 30bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCA264020.27999378545722753No Hit
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCC261460.277278899877459RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGG257130.2726869254398035Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAG251730.26696021366997913No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTC249750.2648604193543769RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC249740.2648498143325809RNA PCR Primer, Index 2 (100% over 34bp)
GACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA248830.2638847573491475RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGAGACCCGCTGGAATTCTCGGGTGCCAA247830.26282425516955044No Hit
CGCGACCTCAGATCAGACGTGGCGACTGGAATTCTCGGGTGCCAAGGAA240730.255294689694411RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCC235480.24972705325152622RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCC234850.24905893687838RNA PCR Primer, Index 1 (100% over 27bp)
CGGTGCAGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA232650.24672583208326637RNA PCR Primer, Index 1 (97% over 37bp)
CACGGTGAAGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT228570.24239898319051023RNA PCR Primer, Index 1 (97% over 35bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG228150.2419535722750794RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGG225600.23924929171710682Illumina Small RNA Adapter 2 (100% over 21bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA215270.22829430420186872RNA PCR Primer, Index 1 (100% over 28bp)
CCCGAGATTGAGCAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC214830.227827683242846RNA PCR Primer, Index 1 (100% over 33bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA207750.22031932781129848RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT202340.21458201101967814RNA PCR Primer, Index 1 (100% over 30bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA202170.21440172564914664RNA PCR Primer, Index 1 (100% over 28bp)
GCCGAGACTAGAGTCACATCCTGACACTGGAATTCTCGGGTGCCAAGGA198730.2107535981513326RNA PCR Primer, Index 1 (100% over 22bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTC191370.2029483021094979RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG189040.20047733203103663RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTTGGAATTCTCGGGTGCCAA189010.20044551696564872No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCTCGGGTGCCAAGG187990.1993638047424597Illumina Small RNA Adapter 2 (100% over 21bp)
CCACCCCGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA186280.19755034601534865RNA PCR Primer, Index 1 (100% over 32bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC186020.19727461544865338RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCC180200.19110249276339825RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTC168360.17854614695696852RNA PCR Primer, Index 1 (100% over 26bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA164260.17419808802062042RNA PCR Primer, Index 1 (100% over 32bp)
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT164090.1740178026500889RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACT163910.17382691225776142RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTC162640.1724800744896731RNA PCR Primer, Index 1 (100% over 26bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCC158380.16796233520458942RNA PCR Primer, Index 1 (100% over 27bp)
AACCAATGATGTAATGATTCTGCCAAATTGGAATTCTCGGGTGCCAAGG157840.167389664027607Illumina Small RNA Adapter 2 (100% over 21bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC157830.16737905900581104RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGAGACCCGCTTGGAATTCTCGGGTGCCA153630.1629249498515032No Hit
ACGAGAACTTTGAAGGCCGAAGTTGGAATTCTCGGGTGCCAAGGAACTC143440.15211843264140867RNA PCR Primer, Index 1 (100% over 26bp)
CCGAGATTGAGCAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC142790.15142910622467057RNA PCR Primer, Index 2 (100% over 34bp)
GACTCTTAGCGGTGGATCACTCGGCTGGCATTCTCGGGTGCCAAGGAAC133050.14109981499539476RNA PCR Primer, Index 1 (95% over 24bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG132330.14033625342608486Illumina Small RNA Adapter 2 (100% over 21bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTC131850.13982721237987825RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA130500.13839553443742214RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC125450.13303999843045677RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCC124200.1317143707059604RNA PCR Primer, Index 1 (100% over 27bp)
AGTTGGTCCGAGTGTTGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC123990.131491665248245RNA PCR Primer, Index 1 (100% over 26bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA122200.1295933663467662No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAA119830.12707997618112105No Hit
TACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACT119640.1268784807669976RNA PCR Primer, Index 1 (100% over 25bp)
TTGGAGGGCAAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC119170.12638004474258696RNA PCR Primer, Index 1 (97% over 36bp)
TTTGGCAATGGTAGAACTCACACTTGGACTTCTCGGGTGCCAAGGAACT117170.12425904038339275RNA PCR Primer, Index 1 (96% over 25bp)
CGGTGGATCACTCGGCTCGTGCGTCGATTGGAATTCTCGGGTGCCAAGG116870.12394088972951361Illumina Small RNA Adapter 2 (100% over 21bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTC116060.12308188296403996RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGT113900.12079119825611022RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCA113860.12074877816892633RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC107930.11446000024391549RNA PCR Primer, Index 1 (100% over 26bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA107750.11426910985158803RNA PCR Primer, Index 1 (100% over 28bp)
CACGGTGAAGAGACATGAGAGGTTGGAATTCTCGGGTGCCAAGGAACTC106160.11258291138602863RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA105180.11154361925002346RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCC101270.10739705572779878RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC98260.1042049441672115RNA PCR Primer, Index 1 (100% over 31bp)
CCGGACACGGACAGGATTGACAGATTTGGAATTCTCGGGTGCCAAGGAA95210.10097041251944033RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph