FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000223780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000223780
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences8348737
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGC426730151.11313244146989No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCC93140511.156238362760739No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGC3920404.6958000952718955No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2097102.5118769461776074No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGC1755402.10259348210394No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCC1604391.9217158236030194No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCC1491621.7866415003850282No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGC1366321.636558919031705No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCA1019271.2208673000478996No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGC888501.064232829468697No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGC788500.9444542330175211No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGC571610.6846664351745659No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCC402710.48236038576853No Hit
TCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCC389480.4665136774580395No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGC366600.4391083345900104No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGGGCC352910.4227106447358445No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGT330230.3955448590607178No Hit
GTTTCCATAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGC316340.37890761201364953No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAA267960.3209587270505706No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCC233380.27953928839775405No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA231700.2775270079773743No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTTGGAATTCTCGGGTGC202490.24253967995398587No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGC181760.21770957690965712No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGTTGC172850.20703730396585737No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGC171980.2059952301767321No Hit
GTTTCCGTAGTGTAGTGGTTATAACGTTCGCCTTGGAATTCTCGGGTGC171580.2055161157909274No Hit
TTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGC161610.1935741897247452No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAA158510.18986105323475874No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTG155240.1859442931308053No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGTGGAATTCTCGGGTGCC154210.18471057358735818No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCA149450.17900911239628223No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGC148600.17799099432644722No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC146100.17499652941516783No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGCATTCTCGGGTGC130850.15673029345636352No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCGTGGAATTCTCGGGTGC125050.14978313486219533No Hit
GTTTCCGTAGTGTAGTGGTTATCACCTTCGCCTTGGAATTCTCGGGTGC114730.13742198370843398No Hit
GCATGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA109470.13112162953510215No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCC107250.12846254469388602No Hit
GTTTCCCTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGC98030.11741895810108763No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC96210.11523898764567622RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA88730.10627954863112828No Hit
TCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA85810.10278201361475395No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC84240.10090148965047048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)