FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000228683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000228683
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences667326
Sequences flagged as poor quality0
Sequence length5-6
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGG27020.404899554340757No Hit
CTTTTT26880.4028016291887323No Hit
GTTTTT24150.3618920887242517No Hit
CTTTG23570.3532006845230068No Hit
CTTGG22590.3385152084588342No Hit
ATTTTT22090.3310226186301748No Hit
CTTTT19930.2986546305703659No Hit
CTGGC17740.2658370871208375No Hit
CCTGG17120.25654627573329974No Hit
CTTTTC16880.2529498326155432No Hit
CTTCTT16840.25235042542925046No Hit
GTTTT16810.25190087003953093No Hit
ATTTT16350.2450076873971642No Hit
CTTCC15700.2352673206199069No Hit
CTTTCT15530.2327198400781627No Hit
GTTTG15360.23017235953641849No Hit
CTCAGG15270.2288236933672598No Hit
CTCCTT15090.2261263610289424No Hit
CTCTG14910.22342902869062495No Hit
CCAGG14840.22238006611461264No Hit
CTTTGG14450.21653584604825826No Hit
CTTCCT14260.2136886619133677No Hit
CTCCC14130.21174058855791622No Hit
CTTCTC14080.2109913295750503No Hit
CTCCAC14070.2108414777784771No Hit
CTCAC14040.21039192238875754No Hit
CTCCTC13900.2082939972367329No Hit
CTTCAT13860.20769459005044014No Hit
CTCAGC13580.20349873974639085No Hit
TTTTTT13380.20050170381492702No Hit
CTCCTG13360.20020200022178064No Hit
CTTTAT13300.1993028894423415No Hit
CTTGGC13110.19645570530745093No Hit
GTTTTC13070.19585629812115818No Hit
CTTCAC12930.19375837296913354No Hit
CTTTC12930.19375837296913354No Hit
CTGGGC12870.1928592621896944No Hit
CTTTGC12860.1927094103931212No Hit
CTTTTG12760.1912108924273893No Hit
GTTTTG12700.19031178164795018No Hit
CTCCAT12550.18806400469935236No Hit
CCCAGG12520.18761444930963278No Hit
CTTCTG12380.1855165241576081No Hit
CAGGG12340.18491711697131535No Hit
ATTTG12330.18476726517474218No Hit
CTGGCT12330.18476726517474218No Hit
CCCAC11720.17562630558377762No Hit
CTCATT11670.1748770466009117No Hit
CTGAC11650.1745773430077653No Hit
CTGCC11630.17427763941461893No Hit
CCCAGC11610.17397793582147256No Hit
CTGGAT11500.1723295660591675No Hit
CAAGG11440.17143045527972833No Hit
CACAC11390.1706811962968624No Hit
CTGGGG11330.16978208551742327No Hit
CTTGGG11280.1690328265345573No Hit
CTTTGT11060.16573608700994719No Hit
CTGAGG11050.16558623521337398No Hit
CTGGTT11030.16528653162022758No Hit
CTGCTT10830.1622894956887638No Hit
CTGCTG10750.1610906813161783No Hit
CCTGGC10750.1610906813161783No Hit
GTTTCT10580.1585432007744341No Hit
GTTTC10450.15659512741898263No Hit
CTCATC10380.15554616484297032No Hit
CTTCT10360.15524646124982394No Hit
ATTTTC10130.15179986992864056No Hit
CTTGTT10030.15030135196290867No Hit
CTTGC9990.14970194477661591No Hit
CTTTCC9930.14880283399717678No Hit
CTGAAT9930.14880283399717678No Hit
CTCTGG9910.1485031304040304No Hit
CTTGGT9900.14835327860745723No Hit
CTGGCC9880.14805357501431085No Hit
CTCCT9830.1473043160314449No Hit
CTGGGT9810.14700461243829852No Hit
ATTTTG9810.14700461243829852No Hit
GTTTGG9760.14625535345543259No Hit
CTCAT9700.14535624267599345No Hit
CTCGG9690.14520639087942025No Hit
CTCAGT9660.1447568354897007No Hit
GTTTAT9640.14445713189655432No Hit
CTTCCC9620.14415742830340794No Hit
CCAGC9620.14415742830340794No Hit
CTGGAC9520.14265891033767603No Hit
CTCACT9450.14160994776166372No Hit
CTGCAT9430.14131024416851734No Hit
CTTGT9400.14086068877879776No Hit
GTTGG9330.13981172620278545No Hit
CTGTG9310.13951202260963907No Hit
CCTGGG9310.13951202260963907No Hit
CTCTGC9290.1392123190164927No Hit
CTGAAC9250.13861291183019994No Hit
CTGATT9180.1375639492541876No Hit
CTGGTG9150.13711439386446805No Hit
GTTTGT9140.13696454206789485No Hit
GTTCTT9110.1365149866781753No Hit
CTTGCT9070.13591557949188254No Hit
CTGGT9040.13546602410216296No Hit
CTTGTC8920.1336678025432847No Hit
CCCACC8910.13351795074671152No Hit
CTTGAT8860.13276869176384554No Hit
CTGCAC8710.13052091481524772No Hit
GGAGG8650.12962180403580859No Hit
CTCTTC8650.12962180403580859No Hit
CTCATG8590.12872269325636945No Hit
TTTTT8550.1281232860700767No Hit
CTGGTC8540.1279734342735035No Hit
CCACC8540.1279734342735035No Hit
CTCCCT8490.12722417529063756No Hit
CTGAGC8450.1266247681043448No Hit
CAGGC8380.12557580552833247No Hit
GTGGG8370.1254259537317593No Hit
CTTAC8290.12422713935917379No Hit
CTGACT8290.12422713935917379No Hit
CTGCCT8230.12332802857973466No Hit
CACAGG8180.12257876959686868No Hit
CTGCTC8100.12137995522428317No Hit
CTCTTT8080.1210802516311368No Hit
CTAGG8060.12078054803799042No Hit
CCCCAC8050.12063069624141723No Hit
CCAAC7980.11958173366540491No Hit
ATTTAT7970.11943188186883172No Hit
GCCAGG7960.11928203007225853No Hit
CCTTG7950.11913217827568534No Hit
CCGGG7900.1183829192928194No Hit
CGGGG7870.11793336390309983No Hit
CTCTGT7870.11793336390309983No Hit
CCTGC7840.11748380851338026No Hit
TTTTG7820.11718410492023389No Hit
CTCTC7790.11673454953051432No Hit
CAGAAT7790.11673454953051432No Hit
GGAAC7760.11628499414079475No Hit
GGATG7760.11628499414079475No Hit
CTTATT7670.11493632797163605No Hit
CCTGCC7670.11493632797163605No Hit
ATTTCT7670.11493632797163605No Hit
GGAAAT7670.11493632797163605No Hit
CTGCT7640.11448677258191649No Hit
ATTTC7610.11403721719219692No Hit
CCCATC7540.1129882546161846No Hit
CAAATT7510.11253869922646503No Hit
CCCTG7480.11208914383674547No Hit
GGAAGG7460.11178944024359908No Hit
CACAGC7430.11133988485387951No Hit
CTCAAC7430.11133988485387951No Hit
CTCAAT7410.11104018126073313No Hit
GTTTGC7340.10999121868472081No Hit
CTTCG7330.10984136688814762No Hit
CCAGCT7290.10924195970185487No Hit
GCCAC7280.10909210790528168No Hit
CCTCAT7250.10864255251556211No Hit
CCCACT7240.10849270071898892No Hit
CTTGAC7230.10834284892241573No Hit
GTCTG7170.1074437381429766No Hit
GTCATT7150.10714403454983022No Hit
AAATG7150.10714403454983022No Hit
CTTGCC7130.10684433095668384No Hit
CCTCC7060.10579536838067152No Hit
GTTCAT6980.10459655400808601No Hit
CCCAGT6970.10444670221151282No Hit
CTTAAT6960.10429685041493963No Hit
CAGCC6940.10399714682179326No Hit
ATTTGG6930.10384729502522007No Hit
CCTCAC6930.10384729502522007No Hit
CTGAT6890.10324788783892731No Hit
CCTCCT6820.10219892526291499No Hit
CTTGTG6770.10144966628004905No Hit
GTTCTG6730.10085025909375626No Hit
CTCCCC6730.10085025909375626No Hit
CAGCTT6720.10070040729718308No Hit
CTGATG6680.10010100011089032No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)