FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000228739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000228739
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences419589
Sequences flagged as poor quality0
Sequence length5-6
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGG17150.4087333080705166No Hit
CTTTTT14840.35367943392224294No Hit
GTTTTT14140.3369964417560994No Hit
CTTGG14040.33461315716093604No Hit
ATTTTT13670.3257950041588316No Hit
CTTTG13060.31125696812833514No Hit
CCTGG12140.2893307498528322No Hit
GTTTT10640.25358148092538174No Hit
CTGGC10510.25048321095166937No Hit
CTTTT10170.24238004332811394No Hit
ATTTT9820.23403854724504217No Hit
TTTTTT9550.2276036788381011No Hit
CTCAGG9510.22665036500003574No Hit
CTTCTT9440.2249820657834214No Hit
CCAGG9380.2235520950263234No Hit
CTTTTC9260.22069215351212734No Hit
CTCTG9230.21997716813357834No Hit
GTTTG9220.21973883967406202No Hit
CTTCC9110.2171172266193823No Hit
CTTTCT8710.20758408823872884No Hit
CTCCAC8670.20663077440066352No Hit
CAGGG8620.20543913210308182No Hit
CTCCC8620.20543913210308182No Hit
CCCAGG8540.20353250442695114No Hit
CTCCTC8530.20329417596743482No Hit
CTTTGG8470.20186420521033677No Hit
CTCAC8410.20043423445323874No Hit
CTGGGC8350.19900426369614072No Hit
CTCAGC8240.19638265064146104No Hit
GTTTTC8160.19447602296533034No Hit
CCCAGC8150.19423769450581402No Hit
CTTCCT8030.191377752991618No Hit
CCCAC7930.18899446839645462No Hit
CTCCTG7800.18589619842274224No Hit
CTGGCT7780.18541954150370957No Hit
CTTTAT7750.18470455612516057No Hit
CTCCTT7750.18470455612516057No Hit
CTTCTC7690.18327458536806254No Hit
CTTCAC7680.18303625690854622No Hit
CTTCAT7620.1816062861514482No Hit
CCTGGC7550.17993798693483387No Hit
CTTGGC7450.1775547023396705No Hit
CTGGGG7440.17731637388015414No Hit
CTCCAT7260.1730264616088601No Hit
CTTGGG7170.17088150547321307No Hit
GTTTTG7130.16992819163514772No Hit
CTGGTT7110.16945153471611504No Hit
CTTCTG7030.16754490703998437No Hit
CTTTGC7030.16754490703998437No Hit
ATTTG6980.1663532647424027No Hit
CTGAGG6950.16563827936385367No Hit
CACAC6930.16516162244482102No Hit
CTTTC6920.16492329398530467No Hit
GTTTGG6900.164446637066272No Hit
CAAGG6830.16277833784965764No Hit
CTGCTG6810.16230168093062497No Hit
CTTTTG6760.16111003863304327No Hit
CTCATT6670.15896508249739627No Hit
CTGGAT6580.15682012636174925No Hit
CTTTGT6560.15634346944271654No Hit
CTTCCC6460.1539601848475532No Hit
GGAGG6410.15276854254997152No Hit
CCAGC6350.1513385717928735No Hit
GTTTCT6330.15086191487384082No Hit
CTGAC6190.14752531644061212No Hit
CTGCC6180.1472869879810958No Hit
CTCATC6160.14681033106206312No Hit
GTTTC6160.14681033106206312No Hit
CTTCT6140.14633367414303045No Hit
CTTGTT6050.14418871800738342No Hit
CTGGCC6040.14395038954786707No Hit
CTCTGC6000.14299707570980175No Hit
GTTTAT5990.1427587472502854No Hit
CCTGGG5970.14228209033125272No Hit
CTCCT5950.14180543341222007No Hit
CTGCTT5870.13989880573608937No Hit
CCCCAC5830.13894549189802402No Hit
CTTGT5830.13894549189802402No Hit
CGGGG5790.13799217805995867No Hit
CTGGAC5780.13775384960044232No Hit
CTGTG5740.136800535762377No Hit
CTGCAT5710.136085550383828No Hit
CTCGG5680.13537056500527897No Hit
CCCACC5670.13513223654576265No Hit
GTGGG5660.1348939080862463No Hit
GCCAGG5650.13465557962672997No Hit
GTTGG5630.1341789227076973No Hit
CTCACT5610.13370226578866462No Hit
ATTTTC5570.13274895195059927No Hit
CTTGGT5560.13251062349108295No Hit
CTTGC5530.13179563811253392No Hit
CTCTGG5490.1308423242744686No Hit
CTTTCC5480.13060399581495225No Hit
CTGGGT5420.12917402505785422No Hit
ATTTTG5420.12917402505785422No Hit
CTGAGC5420.12917402505785422No Hit
CCTGC5390.12845903967930522No Hit
CTTGTC5380.1282207112197889No Hit
CCTTG5370.12798238276027255No Hit
GTTTGT5350.12750572584123987No Hit
CTGCAC5340.12726739738172355No Hit
CTGGTG5340.12726739738172355No Hit
CCACC5330.12702906892220722No Hit
CAGGC5300.1263140835436582No Hit
CTCAT5300.1263140835436582No Hit
GTTCTT5280.12583742662462552No Hit
CTTGAT5260.12536076970559284No Hit
CTCAGT5240.12488411278656018No Hit
CTGGTC5180.12345414202946216No Hit
TTTTT5170.12321581356994583No Hit
CTAGG5160.1229774851104295No Hit
CCTCC5150.12273915665091314No Hit
CTTAC5140.12250082819139682No Hit
GGATG5130.12226249973188048No Hit
GGAAGG5040.12011754359623347No Hit
CCCACT5030.11987921513671712No Hit
CCTGCC5020.11964088667720078No Hit
CACAGG5000.11916422975816812No Hit
CCGGG4960.11821091592010277No Hit
CTGAAT4940.1177342590010701No Hit
CCTCAC4920.11725760208203743No Hit
CTGATT4880.11630428824397207No Hit
CTGGT4870.11606595978445573No Hit
GGGAGG4840.11535097440590672No Hit
CTGACT4840.11535097440590672No Hit
CCCTG4830.1151126459463904No Hit
ATTTAT4830.1151126459463904No Hit
CCTCAT4830.1151126459463904No Hit
TTTTG4800.11439766056784138No Hit
GTCAGG4760.11344434672977603No Hit
CTCTTC4760.11344434672977603No Hit
CTCTGT4760.11344434672977603No Hit
CCTCCT4720.1124910328917107No Hit
CTCTTT4710.11225270443219436No Hit
GGGGG4710.11225270443219436No Hit
CTGCCT4690.1117760475131617No Hit
GCCAC4690.1117760475131617No Hit
GGAAAT4690.1117760475131617No Hit
CTGCTC4670.11129939059412901No Hit
CTTGCT4640.11058440521558No Hit
CACAGC4620.11010774829654733No Hit
CTCCCT4620.11010774829654733No Hit
GCTGG4600.10963109137751466No Hit
GGAAC4590.10939276291799832No Hit
CCAGCC4560.10867777753944931No Hit
CCAGCT4560.10867777753944931No Hit
ATTTC4550.10843944907993298No Hit
CTCATG4540.10820112062041665No Hit
CCCATC4530.10796279216090032No Hit
GTCAC4520.10772446370138397No Hit
CTGAAC4510.10748613524186763No Hit
GTTCAT4500.10724780678235131No Hit
CTCCCC4460.10629449294428596No Hit
CAGAAT4450.10605616448476964No Hit
CCAAC4440.10581783602525328No Hit
AAATG4410.10510285064670427No Hit
CCCAGT4410.10510285064670427No Hit
CTCAAT4390.1046261937276716No Hit
CTTGCC4370.10414953680863892No Hit
CTCTC4360.10391120834912258No Hit
CCTGTC4340.10343455143008992No Hit
CAGCTT4320.10295789451105725No Hit
CACCAC4320.10295789451105725No Hit
CCAGGC4310.10271956605154091No Hit
CCCCAT4290.10224290913250823No Hit
CAGCC4280.1020045806729919No Hit
ATTTGG4280.1020045806729919No Hit
CAAACT4230.10081293837541022No Hit
GTAAAT4200.10009795299686121No Hit
GCCAGC4200.10009795299686121No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)