FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000228795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000228795
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences562813
Sequences flagged as poor quality0
Sequence length5-6
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGG21800.38734002235200676No Hit
CTTTTT21630.3843194808932985No Hit
GTTTTT19750.35091584593817127No Hit
CTTTG19390.34451940520208313No Hit
CTTGG18370.3263961564498332No Hit
ATTTTT17700.31449166952433577No Hit
CTTTT15210.27024962109972583No Hit
GTTTT14740.26189871236094403No Hit
CCTGG14140.25123797780079704No Hit
CTTCTT13940.24768439961408142No Hit
CTTTTC13850.24608528943005936No Hit
CTGGC13790.24501921597404464No Hit
CTTTCT12940.22991650868050312No Hit
ATTTT12880.22885043522448842No Hit
CTTCC12750.22654060940312323No Hit
CTCTG12290.21836737957367722No Hit
CCAGG12250.2176566639363341No Hit
CTTCAT12230.21730130611766252No Hit
CTCAGG12100.21499148029629736No Hit
CTTCCT11900.2114379021095817No Hit
GTTTG11850.21054950756290275No Hit
CTCCTT11740.20859503956020914No Hit
CTTTGG11630.20664057155751556No Hit
CTCCAC11580.20575217701083665No Hit
CTCCTC11530.2048637824641577No Hit
GTTTTC11410.20273163555212834No Hit
CTCAC11080.19686823154404748No Hit
CTTCAC11030.1959798369973686No Hit
TTTTTT10930.19420304790401074No Hit
CTCCTG10840.1926039377199887No Hit
CTTTGC10790.1917155431733098No Hit
CTCCC10780.19153786426397398No Hit
CTTCTC10700.19011643298928774No Hit
CTTTC10660.18940571735194459No Hit
CTTTTG10600.18833964389592991No Hit
GTTTTG10500.18656285480257206No Hit
CTCCAT10500.18656285480257206No Hit
CTTTAT10470.18602981807456473No Hit
CCCAGG10190.1810548086131628No Hit
CTTGGC10130.1799887351571481No Hit
CTCAGC10110.17963337733847656No Hit
ATTTG10070.1789226617011334No Hit
CAGGG9970.1771458726077756No Hit
CCCAC9970.1771458726077756No Hit
CTCATT9740.17305925769305258No Hit
CTGGGC9730.1728815787837168No Hit
CTGGGG9620.17092711078102318No Hit
CTGAC9570.17003871623434427No Hit
CCCAGC9540.1695056795063369No Hit
GTTTAT9470.16826192714098645No Hit
CTGGAT9410.16719585368497172No Hit
CTTCTG9330.16577442241028548No Hit
GTTTGG9320.1655967435009497No Hit
CTTTGT9280.16488602786360657No Hit
GTTTCT9270.16470834895427078No Hit
CTGGTT9140.1623985231329056No Hit
CTGGCT9120.16204316531423404No Hit
CTTCCC8990.15973333949286886No Hit
CACAC8880.15777887149017525No Hit
CTGAGG8780.15600208239681743No Hit
ATTTTG8730.1551136878501385No Hit
CTTCT8670.15404761439412382No Hit
CTGCC8660.15386993548478803No Hit
CAAGG8660.15386993548478803No Hit
CTTGGG8600.15280386202877333No Hit
ATTTTC8580.15244850421010175No Hit
CCAGC8560.15209314639143018No Hit
CTTGTT8540.15173778857275863No Hit
CTGCTT8360.14853956820471453No Hit
CTGCTG8270.1469404580206925No Hit
GTTTC8240.14640742129268514No Hit
GTTGG8210.14587438456467777No Hit
CTCAT8150.1448083111086631No Hit
CTTGGT8150.1448083111086631No Hit
CTCATC8140.1446306321993273No Hit
CCTGGC8020.14249848528729792No Hit
CTCCT8010.14232080637796213No Hit
CTTGC7990.14196544855929058No Hit
CTGTG7920.14072169619394007No Hit
GTTCTT7890.14018865946593273No Hit
CTCACT7840.13930026491925382No Hit
CTTGAT7830.13912258600991803No Hit
CTGATT7750.13770115473523178No Hit
CTTTCC7740.137523475825896No Hit
CTGGGT7720.13716811800722442No Hit
CTTGT7700.13681276018855287No Hit
CTCGG7680.1364574023698813No Hit
CTCAGT7620.1353913289138666No Hit
CTGAAT7610.1352136500045308No Hit
GGAAAT7580.13468061327652348No Hit
GTTTGT7520.13361453982050875No Hit
CTGGT7510.133436860911173No Hit
CTGGAC7500.1332591820018372No Hit
CAGGC7410.13166007181781514No Hit
GTGGG7390.1313047139991436No Hit
CTGAAC7350.13059399836180047No Hit
CCTGGG7290.12952792490578574No Hit
CTGGTC7280.12935024599644998No Hit
CCACC7250.12881720926844262No Hit
CGGGG7210.1281064936310995No Hit
CTGAGC7210.1281064936310995No Hit
GGAGG7190.12775113581242792No Hit
CTCTGC7150.1270404201750848No Hit
CTCTGG7140.12686274126574903No Hit
CTGCAT7060.12544130999106276No Hit
TTTTT7060.12544130999106276No Hit
CTCTTC7030.1249082732630554No Hit
CTCAAT7000.12437523653504805No Hit
CTTGCT6990.12419755762571227No Hit
ATTTAT6960.12366452089770492No Hit
GGATG6910.122776126351026No Hit
CTGGCC6900.12259844744169023No Hit
CTGGTG6840.12153237398567553No Hit
CCCATC6840.12153237398567553No Hit
CCCACC6830.12135469507633975No Hit
ATTTC6820.12117701616700396No Hit
CAGAAT6820.12117701616700396No Hit
GCCAC6800.12082165834833239No Hit
GCCAGG6780.12046630052966083No Hit
CTTGTC6780.12046630052966083No Hit
CTGCAC6740.11975558489231769No Hit
CCCACT6720.11940022707364613No Hit
CTAGG6710.11922254816431035No Hit
CCCCAC6680.118689511436303No Hit
TTTTG6680.118689511436303No Hit
CCTTG6660.11833415361763144No Hit
CTGACT6660.11833415361763144No Hit
CTCCCT6630.11780111688962408No Hit
CCTCC6610.11744575907095252No Hit
CTGCCT6530.11602432779626624No Hit
GGAAC6510.11566896997759468No Hit
CCGGG6500.11549129106825891No Hit
CTCTGT6490.11531361215892312No Hit
CTCTC6480.11513593324958733No Hit
CTTAC6480.11513593324958733No Hit
CCTCAC6460.11478057543091577No Hit
GTCATT6420.11406985979357265No Hit
CTCTTT6380.11335914415622952No Hit
CACAGG6370.11318146524689372No Hit
CACAGC6330.1124707496095506No Hit
CCTGC6280.11158235506287169No Hit
CTTAAT6220.11051628160685699No Hit
CCTCAT6180.10980556596951385No Hit
CTCAAC6140.10909485033217073No Hit
CAGCC6120.10873949251349917No Hit
CTCATG6110.10856181360416337No Hit
GTTCAT6090.1082064557854918No Hit
GTCAC6080.10802877687615602No Hit
ATTTCT6080.10802877687615602No Hit
CCTCCT6080.10802877687615602No Hit
CCAAC6060.10767341905748445No Hit
CTTCG6050.10749574014814868No Hit
GGAAGG6050.10749574014814868No Hit
CAAATT6030.10714038232947712No Hit
CTTGAC6010.10678502451080553No Hit
GTAAAT6000.10660734560146977No Hit
CCCAGT6000.10660734560146977No Hit
CTTATT5960.10589662996412663No Hit
CTTTAC5960.10589662996412663No Hit
CCCTG5940.10554127214545506No Hit
CTGAT5920.1051859143267835No Hit
CAGCAT5910.10500823541744772No Hit
CCCATT5910.10500823541744772No Hit
GTGAC5870.10429751978010457No Hit
CTGCT5860.10411984087076881No Hit
CCAGCT5850.10394216196143301No Hit
CTTGTG5830.10358680414276145No Hit
GTCTG5820.10340912523342566No Hit
GTGAAT5790.10287608850541832No Hit
GTTTGC5780.10269840959608253No Hit
AAATG5780.10269840959608253No Hit
CTGCTC5750.10216537286807517No Hit
CTCTCC5730.10181001504940361No Hit
GTTCTG5720.10163233614006785No Hit
CTTGCC5700.10127697832139629No Hit
CCCCAT5660.10056626268405314No Hit
CTCTT5660.10056626268405314No Hit
CCTGCC5630.10003322595604577No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)