FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000228851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000228851
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences359973
Sequences flagged as poor quality0
Sequence length5-6
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTT14920.41447553010920263No Hit
CTGGG14300.39725201612343153No Hit
CTTGG13680.3800285021376603No Hit
CTTTG13120.36447177982793155No Hit
GTTTTT12780.3550266269970248No Hit
ATTTTT11760.32669116850430446No Hit
CTTTT10250.28474357799057154No Hit
CTTCTT9510.2641864806527156No Hit
GTTTT9450.2625196889766732No Hit
CTTCC9200.25557472365983No Hit
CCTGG9200.25557472365983No Hit
CTTTTC9140.2539079319837877No Hit
CTGGC8940.2483519597303131No Hit
CTCAGG8940.2483519597303131No Hit
CTCCTT8840.2455739736035758No Hit
CTTCAT8670.24085139718812246No Hit
GTTTG8640.24001800135010123No Hit
CTCTG8630.23974020273742755No Hit
ATTTT8460.23501762632197415No Hit
CTCCAC8450.2347398277093004No Hit
CTTTCT8430.23418423048395298No Hit
CTTCCT8110.22529467487839364No Hit
CTCCTC8000.22223889013898265No Hit
CTTCTC7780.2161273206601606No Hit
CTTTTG7680.2133493345334233No Hit
CTTTAT7660.21279373730807588No Hit
CTCAC7610.21140474424470723No Hit
CCAGG7550.20973795256866487No Hit
CTCAGC7520.2089045567306437No Hit
CTCCAT7490.20807116089262248No Hit
CTTTC7400.20557097337855892No Hit
CTTTGG7390.20529317476588524No Hit
CTCCC7390.20529317476588524No Hit
CTTCTG7300.20279298725182165No Hit
CTTCAC7150.19862600806171576No Hit
CTCCTG7110.1975148136110208No Hit
GTTTTC6970.19362563303358865No Hit
CTTGGC6960.1933478344209149No Hit
CTCATT6910.19195884135754626No Hit
CTTTGC6860.1905698482941776No Hit
ATTTG6810.18918085523080896No Hit
CTGGGC6470.17973570239990222No Hit
CTTCT6470.17973570239990222No Hit
GTTTTG6460.17945790378722848No Hit
CCCAGG6450.17918010517455474No Hit
CTCATC6410.17806891072385983No Hit
CTGCC6370.17695771627316495No Hit
TTTTTT6370.17695771627316495No Hit
CCCAC6330.17584652182247001No Hit
CTTGGG6320.1755687232097963No Hit
CAAGC6310.17529092459712256No Hit
CTGGAT6140.17056834818166916No Hit
CAAGG6130.17029054956899545No Hit
CTGGTT6110.169734952343648No Hit
CTTTGT6100.16945715373097428No Hit
CCCAGC6060.16834595928027937No Hit
CTCAT6010.16695696621691072No Hit
CTTGGT5980.16612357037888953No Hit
GTTTGG5940.16501237592819462No Hit
CTGCTG5940.16501237592819462No Hit
CAGGG5930.16473457731552088No Hit
CTGAC5870.16306778563947852No Hit
CTGGGG5850.16251218841413104No Hit
CTTGTT5840.16223438980145732No Hit
GTTTCT5790.1608453967380887No Hit
CTGCTT5740.15945640367472005No Hit
CTCCT5740.15945640367472005No Hit
CTGGCT5720.1589008064493726No Hit
CTTCCC5690.1580674106113514No Hit
ATTTTG5660.1572340147733302No Hit
CCAGC5560.15445602864659294No Hit
CTAGG5550.1541782300339192No Hit
CTCTTC5550.1541782300339192No Hit
CTGTG5470.15195584113252938No Hit
CACAC5380.14945565361846583No Hit
GTTTAT5360.14890005639311837No Hit
CCCACC5350.14862225778044463No Hit
CCTGGC5340.14834445916777092No Hit
CTTGT5240.14556647304103362No Hit
CTTCG5190.144177479977665No Hit
GGATG5170.14362188275231755No Hit
CTCGG5140.14278848691429635No Hit
CTTGAT5140.14278848691429635No Hit
CTCTGG5110.14195509107627516No Hit
GTTTGT5060.1405660980129065No Hit
CTCTGC5050.1402882994002328No Hit
CTTTCC5040.14001050078755906No Hit
CTTGC5040.14001050078755906No Hit
ATTTTC5020.1394549035622116No Hit
CCCATC4990.1386215077241904No Hit
CTGAAC4980.1383437091115167No Hit
CTGGGT4970.13806591049884295No Hit
CTGAAT4950.1375103132734955No Hit
CTGGCC4910.1363991188228006No Hit
GTTTC4900.13612132021012688No Hit
GTTGG4890.13584352159745317No Hit
CTCTGT4880.13556572298477942No Hit
CTGAGG4870.13528792437210568No Hit
CTGAGC4780.13278773685804213No Hit
CTTAC4760.13223213963269467No Hit
GTTCTT4750.13195434102002093No Hit
CTGCAT4740.13167654240734722No Hit
CTCTC4740.13167654240734722No Hit
CCCACT4740.13167654240734722No Hit
CCAAC4720.13112094518199977No Hit
CTGATT4720.13112094518199977No Hit
CCCCAC4710.13084314656932602No Hit
CTCACT4710.13084314656932602No Hit
CTGGAC4690.13028754934397857No Hit
CTCAGT4680.13000975073130483No Hit
CTCAAC4670.12973195211863112No Hit
CTCCCT4670.12973195211863112No Hit
CTCTTT4660.12945415350595738No Hit
CGGGG4590.1275095632172413No Hit
CAGGC4540.12612057015387265No Hit
CTCATG4500.12500937570317774No Hit
CTTGTC4500.12500937570317774No Hit
CCTGGG4470.12417597986515655No Hit
GTGGG4460.12389818125248284No Hit
CTGCT4440.12334258402713535No Hit
GGAGG4430.12306478541446164No Hit
GGAAAT4410.12250918818911419No Hit
CCCCAT4400.12223138957644045No Hit
CCTCC4400.12223138957644045No Hit
CTGACT4360.12112019512574555No Hit
CTGGTG4350.12084239651307181No Hit
CTGGTC4340.1205645979003981No Hit
ATTTAT4330.12028679928772436No Hit
ATTTC4320.12000900067505062No Hit
CCACC4310.1197312020623769No Hit
CTTAAT4290.11917560483702945No Hit
CCTGC4290.11917560483702945No Hit
CTCAAT4280.11889780622435571No Hit
CCTCAT4270.118620007611682No Hit
TTTTT4240.1177866117736608No Hit
CCTCAC4230.11750881316098707No Hit
AAATG4220.11723101454831336No Hit
CACAGG4210.11695321593563962No Hit
CTTGCT4210.11695321593563962No Hit
CTGCTC4180.11611982009761843No Hit
CCCTG4170.11584202148494471No Hit
CCTTG4160.11556422287227097No Hit
CTGGT4150.11528642425959726No Hit
CTGCCT4110.11417522980890232No Hit
CAGAAT4110.11417522980890232No Hit
CTGCAC4080.11334183397088114No Hit
CTCTCT4080.11334183397088114No Hit
CTTATT4050.11250843813285998No Hit
CCGGG4040.11223063952018623No Hit
GTCATT3990.11084164645681759No Hit
CAGCC3950.10973045200612268No Hit
CACAGC3950.10973045200612268No Hit
GCCAC3950.10973045200612268No Hit
CCCATT3950.10973045200612268No Hit
GTCAGG3930.10917485478077524No Hit
CTTGAC3930.10917485478077524No Hit
CTCCCC3850.1069524658793854No Hit
CTCTT3850.1069524658793854No Hit
ATTTCT3850.1069524658793854No Hit
CCTCCT3850.1069524658793854No Hit
GGAAC3800.10556347281601676No Hit
CTGAT3800.10556347281601676No Hit
GTTCAT3770.10473007697799556No Hit
CTTTAC3730.10361888252730066No Hit
CTTGCC3710.1030632853019532No Hit
CTTGTG3690.10250768807660575No Hit
CTGATG3690.10250768807660575No Hit
CCTGCC3660.10167429223858455No Hit
GTCAC3650.10139649362591083No Hit
GTCTG3650.10139649362591083No Hit
GTTCTG3640.10111869501323711No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)