FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000238332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000238332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20365423
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1516350.7445708345954808No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT757400.3719048703284975Clontech SMART CDS Primer II A (100% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341570.16772055262490743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)