FastQCFastQC Report
Thu 9 May 2024
EGAF00000238779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000238779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23323898
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3159471.3546063355276206No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2260990.9693877069776244Clontech SMART CDS Primer II A (100% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349970.1500478179076242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)