FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000238781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000238781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23315175
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3118811.337673854045702No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1986200.8518915255836595Clontech SMART CDS Primer II A (100% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330350.141688835704643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)