FastQCFastQC Report
Wed 15 Mar 2023
EGAF00000239057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000239057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63349627
Sequences flagged as poor quality0
Sequence length101
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT1698110.26805366983455164Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG817400.12902996256000054Illumina Paired End PCR Primer 2 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)