FastQCFastQC Report
Sat 13 May 2023
EGAF00000256697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000256697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22388461
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG828220.3699316357654061Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT411560.18382683829853247Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG236990.10585363594219362Illumina Paired End Adapter 2 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)