FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000256708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000256708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154932172
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT3293770.2125943215977118Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG2226790.1437267658004562Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2147060.1385806428893284Illumina Paired End PCR Primer 2 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)