FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000278909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273784
Sequences flagged as poor quality0
Sequence length40-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC19724172.04255909768284No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGATTTAC121784.448032025246179No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC47071.7192385237997838No Hit
ATCTAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC42271.5439178330362622No Hit
TTCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC30531.1151126435438155No Hit
ACCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC19410.7089530432749904No Hit
ATCAAAGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGATTTAC15370.5613914618823599No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA13340.4872454197469538No Hit
ATCAAAGTATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC9390.34297110130613917No Hit
AACAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC9170.3349355696461444No Hit
AGCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7980.29147064839435466No Hit
ATCAAAGACTGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7880.28781813400344797No Hit
ATCAACGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7760.2834351167343599No Hit
ATCATAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7590.27722584226981856No Hit
ATCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC6920.25275399585074365No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCGTATTAAAACAAGATTTAC6750.24654472138620226No Hit
ATCCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC6730.24581421850802093No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCACATTAAAACAAGATTTAC5660.2067323145253192No Hit
ATCAAAGAATGGCCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5600.20454080589077522No Hit
ATCGAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5390.19687052566987112No Hit
ATCAAATAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5350.19540951991350847No Hit
ATCAAAGCATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5170.18883499400987638No Hit
ATCAAAGAATGGTCCTGCACCCGTAATATGCATATTAAAACAAGATTTAC5170.18883499400987638No Hit
ATCAAAGAATGGTCCTGCACCAGTAGTATGCATATTAAAACAAGATTTAC5060.18481722817987903No Hit
ATCAAAGAATGGTGCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4840.1767816965198843No Hit
ATCAAAGAATGGTCCTGCACCAGTAATACGCATATTAAAACAAGATTTAC4830.17641644508079363No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGGTTTAC4600.16801566198170823No Hit
ATCAAAGAATGGTCCTGCACTAGTAATATGCATATTAAAACAAGATTTAC4540.1658241533471642No Hit
ATCAAAGAATGGTCCTGCACCGGTAATATGCATATTAAAACAAGATTTAC4440.1621716389562575No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATACTAAAACAAGATTTAC4270.15596236449171608No Hit
ATCAAAGAATGGTCCTGTACCAGTAATATGCATATTAAAACAAGATTTAC3860.14098705548899862No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACACGATTTAC3840.1402565526108173No Hit
TCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC3810.13916079829354527No Hit
ATCAAAGAATGGTCCTGCATCAGTAATATGCATATTAAAACAAGATTTAC3640.13295152382900388No Hit
GTCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3460.12637699792537183No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAGGATTTAC3450.12601174648628116No Hit
ATCAAAGAATGGTCCTGCACAGTAATATGCATATTAAAACAAGATTTACC3350.12235923209537446No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACGAGATTTAC3180.11614995763083305No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGATTTAC3150.11505420331356106No Hit
ATCAAAGAATGGTCCTGAACCAGTAATATGCATATTAAAACAAGATTTAC3110.11359319755719838No Hit
ATGAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3060.11176694036174502No Hit
ATCAAAGAATGGTCCTGCCCCAGTAATATGCATATTAAAACAAGATTTAC3020.11030593460538234No Hit
ATCAAAGATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC3000.10957543172720101No Hit
ATCAAAGAATGGTCCTGCACCAGTAATGTGCATATTAAAACAAGATTTAC2950.10774917453174766No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTGC2890.10555766589720364No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGTCTATCAA2870.10482716301902228No Hit
ATCAAAGAATGGTCCTGCACCAGTACTATGCATATTAAAACAAGATTTAC2830.1033661572626596No Hit
ATCAAAGAATGGACCTGCACCAGTAATATGCATATTAAAACAAGATTTAC2820.10300090582356894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)