FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000278910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391084
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC8146920.831586053124138No Hit
AATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTC7553819.31503206472267No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA390869.994272330241074No Hit
ATCAAAGAATGGTCCTGCACTCCAGTCACCAGTCATTTTCAGCAGGCCT248426.352088042466581No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCT137463.5148459154555027No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGTCTATCAA101692.600208650826932No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGATTTAC59031.5093944012028107No Hit
ATCAAAGAATGGTCCTGCACTAGACAGTCATTTTCAGCAGGCCT58111.4858700432643626No Hit
ATCAAAGAATGGTCCTGCACACGTCTGAACTCCAGTCACCAGTCATTTTCAGCAGGCCT56051.433195937445664TruSeq Adapter, Index 16 (96% over 28bp)
AATATGCATATTAAAGCAAGATTTACCTCTATTGTTGGATCATATTCGTC55581.4211780589336305No Hit
ATCAAAGAATGGTCCTGCACTCCAGTCACAGTCATTTTCAGCAGGCCT41321.0565505108876865No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC32540.8320463123011936No Hit
ATCAAAGAATGGTCCTGCACTCCAGTCATTTTCAGCAGGCCTATCAAAGA31540.8064763580202718No Hit
ATCAAAGAATGGTCCTGCACTCCAGTCCAGTCATTTTCAGCAGGCCT27380.7001053482116374No Hit
AATATGCATATTAAAACAAGATTTACCTCTATNGTTGGATCATATTCGTC25080.6412944533655174No Hit
ATCAAAGAATGGTCCTGCACTCCAGTCATTTTCAGCAGGCCT22700.5804379621769236No Hit
ATCAAAGAATGGTCCTGCACGCCTCGAGAGCACTAGTATGGTGATGGTGA21590.5520553129251005No Hit
ATCAAAGAATGGTCCTGCACTCCAGTCACCCAGTCATTTTCAGCAGGCCT17790.4548894866575979No Hit
ATCAAAGAATGGTCCTGCACTCCAGTCACCAGTCATTTTCAGCAGGCCTA17650.45130969305826885No Hit
ATCAAAGAATGGTCCTGCACACGTCTGAACTCCAGTCATTTTCAGCAGGCCT16650.4257397387773471TruSeq Adapter, Index 3 (96% over 25bp)
GTAATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCG15410.39403299546900405No Hit
ATCAAAGAATGGTCCTGCACCAGTCATTTTCAGCAGGCCT12780.3267840157101799No Hit
ATCAAAGAATGGTCCTGCACACGTCCAGTCATTTTCAGCAGGCCT11760.30070266234363974No Hit
ATTAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA11170.2856163893178959No Hit
ATCAAAGAATGGTCCTGCACTTCAGTCACCAGTCATTTTCAGCAGGCCT8500.21734461138783484No Hit
AATATGCATATTAAAACAAGATTTGCCTCTATTGTTGGATCATATTCGTC7770.19867854476276195No Hit
ATCAAAGAATGGTCCTGCACTAGACAGTCACAGTCATTTTCAGCAGGCCT6790.17361998956745864No Hit
ATCAAAGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGATTTAC6310.1613464115126162No Hit
ATCAAAGAATGGTCCTGCACTAGACAGTCATTTTCAGCAGGCCTATCAAA5760.14728293665810924No Hit
ATCACAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA4810.12299148009123359No Hit
AATATGCATATTAAAACAAGATTTACCCCTATTGTTGGATCATATTCGTC4190.1071381084370621No Hit
AATATGCATATTAAAACAAGATTTCCCTCTATTGTTGGATCATATTCGTC4080.10432541346616071No Hit
ATCAAAGTATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3950.1010013194096409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)