FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000278911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000278911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367015
Sequences flagged as poor quality0
Sequence length39-151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC13931837.95975641322561No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTGGATCATATTCGTC365359.954634006784465No Hit
NNNNNNNNNNNNNNNNCAANNNNTNNNNNNNTTGTTGGATCATATTCGTC206125.616119232183971No Hit
NNNNNNNNNNNNNNNNCANNNNNTNNNNNNNNTGTTGGATCATATTCGTC106202.8936147023963597No Hit
NNNNNNNNNNNNNNNNNANNNNNNNNNNCNANTGTTGGATCATATTCGTC59281.6151928395297195No Hit
NNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNTGTTGGATCATATTCGTC56501.5394466166232987No Hit
NNNNNNNNNNNNNNNNCANNNNNTNNNNCNATTGTTGGATCATATTCGTC56271.5331798427857173No Hit
NNNNNNNNNNNNNNNNCANNNNNTNNCNNNNTTGTTGGATCATATTCGTC55041.4996662261760418No Hit
NNTNNNNNNNNNNNNNCAANNNTTNNNNCNATTGTTGGATCATATTCGTC53881.468059888560413No Hit
NNTNNNNNNNNNNNNNCAANNNNTANCNNNNTTGTTGGATCATATTCGTC50951.3882266392381781No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGATCATATTCGTC49561.3503535277849679No Hit
NNNNNNNNNNNNNNNNNANNNNNNNNNNNNANTNTTGGATCATATTCGTC48631.3250139640069207No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNGGATCATATTCGTC45961.2522648938054304No Hit
NNNNNNNNNNNNNNNNCAANNNGTNNNNNNATTGTTGGATCATATTCGTC45811.2481778673896162No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNGGATCATATTCGTC44671.2171164666294294No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGATCATATTCGTC43351.1811506341702656No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNCNNNANTNTTGGATCATATTCGTC31630.8618176368813265No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNTGGATCATATTCGTC24450.6661853057776931No Hit
NANNNNNNNNNNNNNACAANNNTTANNNNTNTTGTTGGATCATATTCGTC24400.6648229636390883No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNTGGATCATATTCGTC22110.6024276936909936No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCTATCAA17910.4879909540481997No Hit
ATCAAAGAATGGTCCTGCACCAGTAATCTGCATATTAAAACAAGATTTAC17270.47055297467405965No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNTGGATCATATTCGTCCA14520.3956241570508018No Hit
ATCAAAGTATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC12450.3392231925125676No Hit
NTNNNNNNNNNNNNNACAANNNTTANNNNTNTTGTTGGATCATATTCGTC11330.30870672860782256No Hit
ATCAAAGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGATTTAC10730.2923586229445663No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATCTTAAAACAAGATTTAC10550.2874541912455894No Hit
CTCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC10210.27819026470307756No Hit
NNNNNNNNNNNNNNNNAAANNNNTNNNNNNNTTGTTGGATCATATTCGTC9730.2651117801724725No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNGTTGGATCATATTCGT7720.210345626200564No Hit
NNNNNNNNNNNNNNNNCAANNNTTNNNNNNATTGTTGGATCATATTCGTC7530.2051687260738662No Hit
ATCAAAGAATGGTACTGCACCAGTAATATGCATATTAAAACAAGATTTAC7440.20271651022437773No Hit
ATTAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7260.1978120785254009No Hit
NNNNNNNNNNNNNNNNCANNNNNTNNNNCNAATGTTGGATCATATTCGTC7210.19644973638679616No Hit
ATCAACGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC7060.19236270997098212No Hit
ATCAAAGAATGGTCCTGAACCAGTAATATGCATATTAAAACAAGATTTAC7030.1915453046878193No Hit
ATCAAAGAATGGTCCTGCACTCCAGTCACCAGTCATTTTCAGCAGGCCT6590.17955669386809803No Hit
ATCAAAGAATGGTNCTGCACTNCNGTCACCAGTCATTTTCAGCAGGCCN6380.17383485688595834No Hit
NNNNNNNNNNNNNNNNCAGNNNNTNNNNNNNTTGTTGGATCATATTCGTC6120.16675067776521396No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAACCAAGATTTAC5750.15666934593953924No Hit
ATCACAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC5650.15394466166232987No Hit
ATCAAAGAATGGTCCTGCACACTAGCAGTCATTTTCAGCAGGCCT5250.14304592455349235No Hit
NNNNNNNNNNNNNNNNACANNNNTNNNNNNNATTGTTGGATCATATTCGT4920.13405446643870142No Hit
ATCAAAGAANGGTCCTGNANACTAGCAGTCATTTTCAGCAGGCCN4900.13350952958325954No Hit
NNTNNNNNNNNNNNNNCAANNNNTTNCNNNNTTGTTGGATCATATTCGTC4880.13296459272781766No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNTGGATCATATTCGTC4860.1324196558723758No Hit
ATCCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4760.1296949715951664No Hit
ATCAAAGAATGGTTCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4760.1296949715951664No Hit
NNNNNNNNNNNNNNNNAANNNNNTNNNNNNNNTGTTGGATCATATTCGTC4650.1266978188902361No Hit
ATCAAAGCATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4590.12506300832391046No Hit
ATCAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACC4480.12206585561898016No Hit
ATCAAAGAATGGTGCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4460.12152091876353828No Hit
ATCAAAGACTGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4420.12043104505265452No Hit
ATCAAAGAATGGCCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4410.1201585766249336No Hit
ATCATAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC4330.11797882920316607No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAACACAAGATTTAC4180.11389180278735202No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCACATTAAAACAAGATTTAC4160.11334686593191014No Hit
ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACACGATTTAC4150.11307439750418921No Hit
NNTNNNNNNNNNNNNNCAANNNTTNNNNCNATTGTTGGCTCATATTCGTC3930.10708009209432856No Hit
ATCAAAGAAAGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTAC3830.10435540781711919No Hit
ATCAAAGAATGGTCCTGTACCAGTAATATGCATATTAAAACAAGATTTAC3700.10081331825674701No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTTGGATCATATTCGTC3680.10026838140130513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)